• Product nameAnti-PADI4 / PAD4 antibody
    See all PADI4 / PAD4 primary antibodies
  • Description
    Rabbit polyclonal to PADI4 / PAD4
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to amino acids 1-15 of human PADI4, conjugated to KLH via a C-terminal added cysteine residue

  • Positive control
    • Extracts of DMSO-treated HL60 cells.



Our Abpromise guarantee covers the use of ab50332 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. 2-4 µl of the antibody immunoprecipitatesPADI4 from DMSO-treated HL60 cells
WB 1/1000 - 1/2000. Predicted molecular weight: 74 kDa.


  • FunctionCatalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1.
  • Tissue specificityExpressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes.
  • Involvement in diseaseGenetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context.
  • Sequence similaritiesBelongs to the protein arginine deiminase family.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC antibody
    • HL 60 PAD antibody
    • HL-60 PAD antibody
    • PAD 4 antibody
    • PAD antibody
    • PADI 4 antibody
    • PADI 5 antibody
    • PADI H protein antibody
    • Padi4 antibody
    • PADI4_HUMAN antibody
    • PADI5 antibody
    • PDI 4 antibody
    • PDI 5 antibody
    • PDI4 antibody
    • PDI5 antibody
    • Peptidyl arginine deiminase type IV antibody
    • Peptidyl arginine deiminase type V antibody
    • Peptidylarginine deiminase IV antibody
    • Protein arginine deiminase antibody
    • Protein arginine deiminase type 4 antibody
    • Protein arginine deiminase type IV antibody
    • Protein-arginine deiminase type IV antibody
    • Protein-arginine deiminase type-4 antibody
    see all

Anti-PADI4 / PAD4 antibody images

  • All lanes : Anti-PADI4 / PAD4 antibody (ab50332) at 1/1000 dilution

    Lane 1 : 293-T cells
    Lane 2 : 293-T cells transfected with PADI4 cDNA
    Lane 3 : HL60 cells
    Lane 4 : HL60 cells induced with 1.25% DMSO for three days

    Goat Anti-Rabbit IgG

    Predicted band size : 74 kDa
    Observed band size : ~62 kDa (why is the actual band size different from the predicted?)

References for Anti-PADI4 / PAD4 antibody (ab50332)

This product has been referenced in:
  • Sharma P  et al. Citrullination of Histone H3 Interferes with HP1-Mediated Transcriptional Repression. PLoS Genet 8:e1002934 (2012). WB . Read more (PubMed: 23028349) »
  • Tanikawa C  et al. Regulation of protein Citrullination through p53/PADI4 network in DNA damage response. Cancer Res 69:8761-9 (2009). WB ; Human . Read more (PubMed: 19843866) »

See all 2 Publications for this product

Product Wall

Thank you for your enquiry. The exact protocols are as below: 1. DMSO was added to a concentration of 1.25% to normal HL60 media. This would be the equivalent to adding 125ul DMSO to 10ml media. No additional buffers were required. 2. The HL...

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