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PADPR mixed with methylated bovine serum albumin.
Our Abpromise guarantee covers the use of ab14459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 - 10 µg/ml. 2 µg/ml, if using ECL or 10 µg/ml, if using colorimetric methods.|
|ELISA||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration. ab18392-Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.|
Image courtesy of Dr Aashish Joshi by Abreview.
ab14459 staining PADPR in Rat cardiomyoblast (H9c2) by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with 0.1% Triton X-100. The sample was incubated with the primary antibody (1/1000 in 3% BSA) for 1 hour at 37°C. ab98707, FITC-conjugated Goat anti-mouse FITC (IgG3 heavy chain preadsorbed 1/1000) was used as the secondary antibody.