Overview

  • Product name
    Anti-PAF Receptor antibody
    See all PAF Receptor primary antibodies
  • Description
    Rabbit polyclonal to PAF Receptor
  • Tested applications
    Suitable for: WB, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide:

    MEPHDSSHMDSEFRYTL

    conjugated to KLH, corresponding to N terminal amino acids 1-17 of Human PAF Receptor (Peptide available as ab107706.)

  • Positive control
    • A549 membrane fraction. U937 and Raji cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab104162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF Receptor peptide (ab107706).
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Images

  • All lanes : Anti-PAF Receptor antibody (ab104162) at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : A549 membrane fraction at 30 µg

    Secondary
    Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 39 kDa
  • PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 39kDa; PAF Receptor

References

This product has been referenced in:
  • Yu Y  et al. The expression of platelet-activating factor receptor modulates the cisplatin sensitivity of ovarian cancer cells: a novel target for combination therapy. Br J Cancer N/A:N/A (2014). WB . Read more (PubMed: 24921917) »
  • Yuan D  et al. Enhanced permeability responses to inflammation in streptozotocin-induced diabetic rat venules: Rho-mediated alterations of actin cytoskeleton and VE-cadherin. Am J Physiol Heart Circ Physiol 307:H44-53 (2014). Read more (PubMed: 24778164) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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