Overview

  • Product nameAnti-PAF Receptor antibody
    See all PAF Receptor primary antibodies
  • Description
    Rabbit polyclonal to PAF Receptor
  • Tested applicationsSuitable for: WB, ICC, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
    Predicted to work with: Rhesus monkey
  • Immunogen

    Synthetic peptide:

    MEPHDSSHMDSEFRYTL

    conjugated to KLH, corresponding to N terminal amino acids 1-17 of Human PAF Receptor (Peptide available as ab107706.)

  • Positive control
    • A549 membrane fraction. U937 and Raji cell lysates.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab104162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200. Predicted molecular weight: 39 kDa.Can be blocked with PAF Receptor peptide (ab107706).
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

Anti-PAF Receptor antibody images

  • All lanes : Anti-PAF Receptor antibody (ab104162) at 1 µg/ml

    Lane 1 : Molecular weight markers
    Lane 2 : A549 membrane fraction at 30 µg

    Secondary
    Anti-rabbit IgG (HRP) conjugate at 1/10000 dilution
    Developed using the ECL technique

    Predicted band size : 39 kDa
  • PAF Receptor was immunoprecipitated using 0.5mg PANC1 whole cell extract, 5µg of Rabbit polyclonal to PAF Receptor and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, PANC1 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104162.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 39kDa; PAF Receptor

References for Anti-PAF Receptor antibody (ab104162)

This product has been referenced in:
  • Yu Y  et al. The expression of platelet-activating factor receptor modulates the cisplatin sensitivity of ovarian cancer cells: a novel target for combination therapy. Br J Cancer N/A:N/A (2014). WB . Read more (PubMed: 24921917) »
  • Yuan D  et al. Enhanced permeability responses to inflammation in streptozotocin-induced diabetic rat venules: Rho-mediated alterations of actin cytoskeleton and VE-cadherin. Am J Physiol Heart Circ Physiol 307:H44-53 (2014). Read more (PubMed: 24778164) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"