Overview

  • Product name
  • Description
    Rabbit polyclonal to PAF49
  • Tested applications
    Suitable for: IHC-P, IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to a region between residues 225 and 275 of human PAF49 (NP_036231.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab75252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
IP
  • Application notes
    IP: Use at 2-5 µg/mg of lysate.

    Is unsuitable for WB.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
      Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
    • Sequence similarities
      Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
    • Post-translational
      modifications
      Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
      Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
    • Information by UniProt
    • Database links
    • Alternative names
      • A34.5 antibody
      • Anti sense to ERCC 1 protein antibody
      • Antisense to ERCC-1 protein antibody
      • ASE 1 antibody
      • ASE-1 antibody
      • ASE1 antibody
      • CAST antibody
      • CD3 epsilon associated protein antibody
      • CD3-epsilon-associated protein antibody
      • CD3E antigen, epsilon polypeptide associated protein antibody
      • CD3E associated protein antibody
      • CD3e molecule, epsilon associated protein antibody
      • CD3E-associated protein antibody
      • CD3EAP antibody
      • DNA directed RNA polymerase I subunit RPA34 antibody
      • DNA-directed RNA polymerase I subunit RPA34 antibody
      • MGC118851 antibody
      • PAF 49 antibody
      • RNA polymerase I associated factor PAF49 antibody
      • RNA polymerase I-associated factor PAF49 antibody
      • RPA34_HUMAN antibody
      see all

    References

    ab75252 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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