Overview

  • Product name
  • Description
    Rabbit polyclonal to PAF49
  • Tested applications
    Suitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide corresponding to a region between residues 404 and 454 of human PAF49 (NP_036231.1)

  • Positive control
    • HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab76933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 55 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC 1/50 - 1/250.

Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

ICC/IF 1/50 - 1/250.

Target

  • Function
    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation.
    Isoform 2 has been described as a component of preformed T-cell receptor (TCR) complex.
  • Sequence similarities
    Belongs to the eukaryotic RPA34 RNA polymerase subunit family.
  • Post-translational
    modifications
    Isoform 2 undergoes tyrosine phosphorylation upon T-cell receptor (TCR) stimulation. This phosphorylation has not been confirmed by other group.
    Isoform 1 is phosphorylated on tyrosine residues in initiation-competent Pol I-beta complexes but not in Pol I-alpha complexes.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Nucleus > nucleolus. Chromosome. Found at the fibrillar centers of the nucleolus in interphase and during cell division it is localized to the nucleolus organizer regions of the chromosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • A34.5 antibody
    • Anti sense to ERCC 1 protein antibody
    • Antisense to ERCC-1 protein antibody
    • ASE 1 antibody
    • ASE-1 antibody
    • ASE1 antibody
    • CAST antibody
    • CD3 epsilon associated protein antibody
    • CD3-epsilon-associated protein antibody
    • CD3E antigen, epsilon polypeptide associated protein antibody
    • CD3E associated protein antibody
    • CD3e molecule, epsilon associated protein antibody
    • CD3E-associated protein antibody
    • CD3EAP antibody
    • DNA directed RNA polymerase I subunit RPA34 antibody
    • DNA-directed RNA polymerase I subunit RPA34 antibody
    • MGC118851 antibody
    • PAF 49 antibody
    • RNA polymerase I associated factor PAF49 antibody
    • RNA polymerase I-associated factor PAF49 antibody
    • RPA34_HUMAN antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PAF49 with ab76933 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PAF49 antibody (ab76933) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 55 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 130 kDa. We are unsure as to the identity of these extra bands.
  • ab76933 at 1µg/ml staining PAF49 in HeLa whole cell lysate immunoprecipitated using ab76933 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

References

ab76933 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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