Recombinant
RabMAb

Anti-PAFAH1B2 antibody [EPR11250] (ab157479)

Overview

  • Product name
    Anti-PAFAH1B2 antibody [EPR11250]
  • Description
    Rabbit monoclonal [EPR11250] to PAFAH1B2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to residues in Human PAFAH1B2 (UniProt P68402).

  • Positive control
    • SH-SY5Y, 293T, Human testis and K562 lysates; K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab157479 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 26 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function
      Inactivates PAF by removing the acetyl group at the sn-2 position. This is a catalytic subunit.
    • Tissue specificity
      Ubiquitous.
    • Sequence similarities
      Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Intracellular platelet activating factor acetylhydrolase alpha 2 subunit antibody
      • PA1B2_HUMAN antibody
      • PAF acetylhydrolase 30 kDa subunit antibody
      • PAF AH 30 kDa subunit antibody
      • PAF AH beta subunit antibody
      • PAF AH subunit beta antibody
      • PAF-AH 30 kDa subunit antibody
      • PAF-AH subunit beta antibody
      • PAFAH 30 kDa subunit antibody
      • PAFAH beta subunit antibody
      • PAFAH subunit beta antibody
      • Pafah1b2 antibody
      • PAFAH1B2 protein antibody
      • PAFAHB antibody
      • Platelet activating factor acetylhydrolase 1b catalytic subunit 2 antibody
      • Platelet activating factor acetylhydrolase IB beta subunit antibody
      • Platelet activating factor acetylhydrolase IB subunit beta antibody
      • Platelet activating factor acetylhydrolase isoform Ib beta subunit antibody
      • Platelet activating factor acetylhydrolase isoform Ib subunit 2 antibody
      • Platelet-activating factor acetylhydrolase IB subunit beta antibody
      see all

    Images

    • All lanes : Anti-PAFAH1B2 antibody [EPR11250] (ab157479) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Human testis lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 26 kDa

    • Immunofluorescent analysis of K562 cells labeling PAFAH1B2 with ab157479 at 1/50 dilution.
    • Flow cytometric analysis of permeabilized K562 cells labeling PAFAH1B2 with ab157479 at 1/100 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab157479 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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