Overview

  • Product nameAnti-PAG antibody
    See all PAG primary antibodies
  • Description
    Rabbit polyclonal to PAG
  • Tested applicationsSuitable for: IP, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 97-432 of Human PAG.

  • Positive control
    • Human Ramos cell line, Raji cell line Mouse spleen cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from rabbit antisera by precipitation methods.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 51.8 kDa. Use a lysis buffer made with Tris HCl (pH 8.1) and 1%laurylmaltoside (with common protease inhibitors), mild detergents such as NP-40 do not work. Non-denaturing conditions should be used

Target

  • FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • Tissue specificityUbiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • Post-translational
    modifications
    Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • Cellular localizationCell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBP antibody
    • Csk binding protein antibody
    • Csk-binding protein antibody
    • FLJ37858 antibody
    • MGC138364 antibody
    • PAG 1 antibody
    • PAG antibody
    • PAG1 antibody
    • PAG1_HUMAN antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
    • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
    • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
    • Transmembrane adapter protein PAG antibody
    • Transmembrane phosphoprotein Cbp antibody
    see all

Anti-PAG antibody images

  • All lanes : Anti-PAG antibody (ab14989) at 1 µg/ml

    Lane 1 : Human liver tissue lysate - total protein (ab29889)
    Lane 2 : Lung (Human) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 51.8 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • IHC image of ab14989 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab14989, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-PAG antibody (ab14989)

This product has been referenced in:
  • Cai H  et al. Differential transformation capacity of Src family kinases during the initiation of prostate cancer. Proc Natl Acad Sci U S A 108:6579-84 (2011). IHC-P ; Mouse . Read more (PubMed: 21464326) »
  • Boyd RS  et al. Protein profiling of plasma membranes defines aberrant signaling pathways in mantle cell lymphoma. Mol Cell Proteomics : (2009). Read more (PubMed: 19346216) »

See all 2 Publications for this product

Product Wall

My apologies for the late reply, I would like to help you as my colleague Tanya is away: Laurylmaltoside is a detergent for cell lysis buffer not for SDS-sample buffer. I have just been informed by the supplier that the antibody should stain also re...

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My apologies for the late reply, I would like to help you as my colleague Tanya is away: Laurylmaltoside is a detergent for cell lysis buffer not for SDS-sample buffer. I have just been informed by the supplier that the antibody should stain also re...

Read More

Thank you for your enquiry. We would like to confirm that this antibody has been tested and characterised for Western blot analysis and it recognizes mouse PAG. Unfortunately, there is no published reference available yet. We will update the on...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"