Overview

  • Product nameAnti-PAG antibody [PAG-C1]
    See all PAG primary antibodies
  • Description
    Mouse monoclonal [PAG-C1] to PAG
  • Tested applicationsSuitable for: IHC-P, IP, WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic C-teminal peptide (last 15 amino acids) (Human) conjugated to KLH.

  • EpitopeLocated in the C-terminal domain.
  • Positive control
    • Human Ramos cell line, Raji cell line Mouse spleen cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from hybridoma culture supernatant by protein A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberPAG-C1
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4206 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Use covalently bound ab4206 to Sepharose beads as immunosorbent. This method of precipitation obviously differs from protein A/G-sepharose or Goat xMs Sepharose mediated immunoprecipitation protocols.

WB Use at an assay dependent concentration.

This antibody can be used for WB, but the signal is very faint, and we do not recommend or guarantee it for this method. We recommend the clone MEM-255 (ab9527) or the rabbit polyclonal ab14989, which are much better for WB.

Flow Cyt Use 1µg for 106 cells.

Target

  • FunctionNegatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling.
  • Tissue specificityUbiquitously expressed. Present in germinal center B-cells, plasma cells, T-cells, monocytes and platelets (at protein level).
  • Post-translational
    modifications
    Palmitoylated.
    Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation.
  • Cellular localizationCell membrane. Present in lipid rafts.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBP antibody
    • Csk binding protein antibody
    • Csk-binding protein antibody
    • FLJ37858 antibody
    • MGC138364 antibody
    • PAG 1 antibody
    • PAG antibody
    • PAG1 antibody
    • PAG1_HUMAN antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid enriched microdomains antibody
    • Phosphoprotein associated with glycosphingolipid microdomains 1 antibody
    • Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 antibody
    • Protein Associated with Glycosphingolipid Enriched Microdomains antibody
    • Transmembrane adapter protein PAG antibody
    • Transmembrane phosphoprotein Cbp antibody
    see all

Anti-PAG antibody [PAG-C1] images

  • Overlay histogram showing Jurkat cells stained with ab4206 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab4206, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
  • 1. Frozen Raji cells lysate, immunoprecipitated with ab4206

    2. Fresh Raji cells lysate, immunoprecipitated with ab4206

    3. Frozen Raji cells lysate, immunoprecipitated with ab14989

    Reprobe with ab9527.

    The immunoprecipitation used covalently bound antibody to Sepharose beads as immunosorbent. This method of precipitation obviously differs from proteinA/G-sepharose or GoatxMs -Sepharose mediated immunoprecipitation protocols.

  • ab4206 staining PAG in human germinal center of lymphatic follicle and in dispersed T cells in appendix tissue section by immunohistochemistry (Formalin/ PFA fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in citrate buffer pH 6.1. Th image show positive signal in T-cells.

References for Anti-PAG antibody [PAG-C1] (ab4206)

This product has been referenced in:
  • Baumgartner M  et al. Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes. Blood 101:1874-81 (2003). Read more (PubMed: 12411311) »
  • Vang T  et al. Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling. J Biol Chem 278:17597-600 (2003). Read more (PubMed: 12665526) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"