Overview

  • Product nameAnti-PAG3 antibody
    See all PAG3 primary antibodies
  • Description
    Rabbit polyclonal to PAG3
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to region within amino acids 900-950 of Human PAG3 (NP_003878.1).

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
    Note: pH 7 to 8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab117760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 112 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • FunctionActivates the small GTPases ARF1, ARF5 and ARF6. Regulates the formation of post-Golgi vesicles and modulates constitutive secretion. Modulates phagocytosis mediated by Fc gamma receptor and ARF6. Modulates PXN recruitment to focal contacts and cell migration.
  • Tissue specificityDetected in heart, brain, placenta, kidney, monocytes and pancreas.
  • Sequence similaritiesContains 2 ANK repeats.
    Contains 1 Arf-GAP domain.
    Contains 1 PH domain.
    Contains 1 SH3 domain.
  • DomainThe conserved Arg-464 in the Arf-GAP domain probably becomes part of the active site of bound small GTPases and is necessary for GTP hydrolysis.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues by SRC and PTK2B.
  • Cellular localizationCytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Colocalizes with F-actin and ARF6 in phagocytic cups.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMAP 2 antibody
    • AMAP2 antibody
    • ANK repeat and PH domain-containing protein 2 antibody
    • Arf-GAP with SH3 domain antibody
    • ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 antibody
    • ASAP2 antibody
    • ASAP2_HUMAN antibody
    • centaurin, beta 3 antibody
    • CENTB3 antibody
    • DDEF 2 antibody
    • DDEF2 antibody
    • Development and differentiation enhancing factor 2 antibody
    • Development and differentiation-enhancing factor 2 antibody
    • FLJ42910 antibody
    • KIAA0400 antibody
    • PAG 3 antibody
    • PAG3 antibody
    • Pap alpha antibody
    • PAP antibody
    • Paxillin associated protein with ARFGAP activity 3 antibody
    • Paxillin-associated protein with ARF GAP activity 3 antibody
    • Pyk2 C terminus associated protein antibody
    • Pyk2 C-terminus-associated protein antibody
    • SHAG 1 antibody
    • SHAG1 antibody
    see all

Anti-PAG3 antibody images

  • All lanes : Anti-PAG3 antibody (ab117760) at 0.1 µg/ml

    Lane 1 : Hela whole cell lysate at 50 µg
    Lane 2 : Hela whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 112 kDa


    Exposure time : 3 minutes
  • Immunoprecipitation : 1 mg of HeLa cell lysate immunoprecipitated with ab117760 at 6 µg/mg lysate; 20% of immunoprecipitate loaded in lane

    Western Blot: PAG3 antibody (ab117760) at 1 µg/ml

    developed using the ECL technique

    Exposure time : 30 seconds

    Predicted band size : 112 kDa

References for Anti-PAG3 antibody (ab117760)

ab117760 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"