Customer reviews and FAQs

Overview

  • Product name
    Anti-PAI1 antibody
    See all PAI1 primary antibodies
  • Description
    Rabbit polyclonal to PAI1
  • Specificity
    Ab20562 recognizes Human PAI-1 both alone and in complex under reducing and native conditions.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein (Human PAI1).

Properties

Applications

Our Abpromise guarantee covers the use of ab20562 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50000.
WB 1/5000. Predicted molecular weight: 45 kDa. Dilution optimised using Chromogenic detection

Target

  • Function
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
    • Alternative names
      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    References for Anti-PAI1 antibody (ab20562)

    This product has been referenced in:
    • Ahmed Z  et al. Decorin blocks scarring and cystic cavitation in acute and induces scar dissolution in chronic spinal cord wounds. Neurobiol Dis 64:163-76 (2014). Read more (PubMed: 24384090) »
    • Wang L  et al. uPA binding to PAI-1 induces corneal myofibroblast differentiation on vitronectin. Invest Ophthalmol Vis Sci 53:4765-75 (2012). WB . Read more (PubMed: 22700714) »

    See all 5 Publications for this product

    Publishing research using ab20562? Please let us know so that we can cite the reference in this datasheet.

    Product Wall

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    3 Abreviews or Q&A

    10
    Votes: Highest First

    Western blot abreview for Anti-PAI1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (jurkat cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    100000 cells
    Specification
    jurkat cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More
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    Abcam user community

    Verified customer

    Submitted Jul 31 2012

    I bought PAI1 antibody about one-two month ago. (I can't remember the lot number)

    I treated HUVEC with two senescence inducers (H2O2 and High Glucose) and detected two bands.

    Experimental condition is normal.

    1:5000 primary a...

    Read More

    Thank you for contacting us.

    Most likely the lower band is for total PAI1 and the upper band is for glycosylated PAI1. This protein gets glycosylated at at least 3 locations according to Swiss Prot, so this post translational modification can ...

    Read More

    Western blot abreview for Anti-PAI1 antibody

     Excellent
    Abreviews
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - other (carcinoma)
    Gel Running Conditions
    Non-reduced Denaturing
    Loading amount
    15 µg
    Specification
    carcinoma
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    Submitted Nov 21 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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