- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-PAI1 antibody
See all PAI1 primary antibodies -
DescriptionRabbit polyclonal to PAI1
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Host speciesRabbit
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SpecificityAb20562 recognizes Human PAI-1 both alone and in complex under reducing and native conditions.
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Tested applicationsSuitable for: ELISA, WBmore details
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Species reactivityReacts with: Rat, Human
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Immunogen
Recombinant full length protein (Human PAI1).
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA. pH 6.6 -
Concentration information loading... -
PurityProtein A purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab20562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/50000. | |
| WB | 1/5000. Predicted molecular weight: 45 kDa. Dilution optimised using Chromogenic detection |
Target
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FunctionThis inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
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Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
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Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. -
Sequence similaritiesBelongs to the serpin family.
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Post-translational
modificationsInactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
-Met-370 bond. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 5054 Human
- Entrez Gene: 24617 Rat
- Omim: 173360 Human
- SwissProt: P05121 Human
- SwissProt: P20961 Rat
- Unigene: 414795 Human
- Unigene: 713079 Human
- Unigene: 29367 Rat
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Alternative names
- Clade E antibody
- Endothelial plasminogen activator inhibitor antibody
- Nexin antibody
see all
Protocols
References
This product has been referenced in:
- Bennett RE et al. Tau induces blood vessel abnormalities and angiogenesis-related gene expression in P301L transgenic mice and human Alzheimer's disease. Proc Natl Acad Sci U S A 115:E1289-E1298 (2018). Read more (PubMed: 29358399) »
- Qin Y et al. Augmented expression of cardiac ankyrin repeat protein is induced by pemetrexed and a possible marker for the pemetrexed resistance in mesothelioma cells. Cancer Cell Int 17:120 (2017). Read more (PubMed: 29238267) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"