• Product name
  • Description
    Rabbit polyclonal to PAI1
  • Host species
  • Tested applications
    Suitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length PAI1 protein (Mouse).

  • Positive control
    • Paraffin sections of lung and carotid.



Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration. PubMed: 18276785
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 1 - 10 µg/ml.


  • Function
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all


  • ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.


This product has been referenced in:
  • Magnussen SN  et al. Cleavage of the urokinase receptor (uPAR) on oral cancer cells: regulation by transforming growth factor - ß1 (TGF-ß1) and potential effects on migration and invasion. BMC Cancer 17:350 (2017). Read more (PubMed: 28526008) »
  • Swaminathan SS  et al. TGF-ß2-mediated ocular hypertension is attenuated in SPARC-null mice. Invest Ophthalmol Vis Sci 55:4084-97 (2014). IHC . Read more (PubMed: 24906856) »

See all 10 Publications for this product

Customer reviews and Q&As

Thanks for your enquiry and for providing the protocol and data.

To our knowledge, this antibody has not previously been tested for reactivity to human PAI-1. It may be there is no reactivity to the human protein. Do you happen to have any mo...

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