• Product nameAnti-PAI1 antibody
    See all PAI1 primary antibodies
  • Description
    Rabbit polyclonal to PAI1
  • Tested applicationsSuitable for: IHC-Fr, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length PAI1 protein (Mouse).

  • Positive control
    • Paraffin sections of lung and carotid.



Our Abpromise guarantee covers the use of ab28207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: Use at a concentration of 1 - 10 µg/ml.
    IHC-Fr: Use at an assay dependent dilution (PMID 18276785).
    WB: Use at a concentration of 1-5 µg/ml. Predicted molecular weight: 45 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
    • Tissue specificityFound in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
    • Involvement in diseaseDefects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
      Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
    • Sequence similaritiesBelongs to the serpin family.
    • Post-translational
      Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
      -Met-370 bond.
    • Cellular localizationSecreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Clade E antibody
      • Endothelial plasminogen activator inhibitor antibody
      • Nexin antibody
      • PAI 1 antibody
      • PAI antibody
      • PAI-1 antibody
      • PAI1_HUMAN antibody
      • PLANH1 antibody
      • Plasminogen activator inhibitor 1 antibody
      • Plasminogen activator inhibitor type 1 antibody
      • Serine (or cysteine) proteinase inhibitor antibody
      • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin E1 antibody
      • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
      • Serpin peptidase inhibitor clade E antibody
      • Serpine 1 antibody
      • SERPINE1 antibody
      see all

    Anti-PAI1 antibody images

    • ab28207 staining PAI1 in E18.5 mouse tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). Subsequent to deparaffinization, the sections were hydrated, heated in 10mM citrate buffer (pH 6.0) and treated with 1% H2O2 in methanol for 10 min and blocked with 10% of normal serum. The primary antibody was incubated with sample at 4°C overnight.

    References for Anti-PAI1 antibody (ab28207)

    This product has been referenced in:
    • Achuta VS  et al. Tissue plasminogen activator contributes to alterations of neuronal migration and activity-dependent responses in fragile X mice. J Neurosci 34:1916-23 (2014). Mouse . Read more (PubMed: 24478370) »
    • Cunha SI  et al. Genetic and pharmacological targeting of activin receptor-like kinase 1 impairs tumor growth and angiogenesis. J Exp Med 207:85-100, S1-5 (2010). WB ; Mouse . Read more (PubMed: 20065063) »

    See all 7 Publications for this product

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    Thanks for your enquiry and for providing the protocol and data.

    To our knowledge, this antibody has not previously been tested for reactivity to human PAI-1. It may be there is no reactivity to the human protein. Do you happen to have any mo...

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