Recombinant
RabMAb

Anti-PAI1 antibody [EPR17272-21] (ab182973)

Overview

  • Product name
    Anti-PAI1 antibody [EPR17272-21]
    See all PAI1 primary antibodies
  • Description
    Rabbit monoclonal [EPR17272-21] to PAI1
  • Tested applications
    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Mouse PAI1 aa 1-250. The exact sequence is proprietary.
    Database link: P22777

  • Positive control
    • WB: Hepa1-6 and HepG2 whole cell lysates; mouse lung (7 days supernatant). ICC/IF: Hepa1-6 cells. Flow cytometry: Hepa1-6 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab182973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/60.
WB 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF 1/100.

Target

  • Function
    This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.
  • Tissue specificity
    Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
  • Involvement in disease
    Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen.
    Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-
    -Met-370 bond.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Clade E antibody
    • Endothelial plasminogen activator inhibitor antibody
    • Nexin antibody
    • PAI 1 antibody
    • PAI antibody
    • PAI-1 antibody
    • PAI1_HUMAN antibody
    • PLANH1 antibody
    • Plasminogen activator inhibitor 1 antibody
    • Plasminogen activator inhibitor type 1 antibody
    • Serine (or cysteine) proteinase inhibitor antibody
    • Serine (or cysteine) proteinase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin E1 antibody
    • Serpin peptidase inhibitor clade E (nexin plasminogen activator inhibitor type 1) member 1 antibody
    • Serpin peptidase inhibitor clade E antibody
    • Serpine 1 antibody
    • SERPINE1 antibody
    see all

Images

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with ab182973 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cells.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).


    -ve control: PBS only, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary at 1/1000 dilution.

  • All lanes : Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/1000 dilution

    Lane 1 : Hepa1-6 (mouse hepatoma epithelial cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa

    Blocking and dilution buffer: 5% NFDM/TBST

    Exposure times.

    Lane 1: 1 second

    Lane 2: 3 seconds

  • Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/1000 dilution + Mouse lung (7 days supernatant) at 10 µl

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 3 seconds

    Blocking and dilution buffer: 5% NFDM/TBST 

  • All lanes : Anti-PAI1 antibody [EPR17272-21] (ab182973) at 1/5000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) grown in serum-free media for 18 hours, whole cell lysate
    Lane 2 : HepG2 grown in serum-free media for 18 hours, then treated with 10ng/ml TGFß for 24 hours, whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 3 minutes

    Blocking and diluting buffer: 5% NFDM/TBST 

    This image was performed on a BIO-RAD® ChemiDoc™ MP instrument. The expression profile observed is consistent with what has been described in the literature (PMID: 20519507).

  • Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methonol-permeabilized Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling PAI1 with ab182973 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

References

ab182973 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab182973.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up