PAI1 overexpression 293T lysate (whole cell) (ab94070)

Overview

  • Product namePAI1 overexpression 293T lysate (whole cell)
    See all PAI1 lysates
  • Description
    PAI1 overexpression lysate
  • General notesab94070 is a 293T cell transfected lysate in which Human PAI1 has been transiently over-expressed using a pCMV-PAI1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundDisease: Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. Function: This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. PTM: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg- -Met-370 bond. Similarity: Belongs to the serpin family. Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.

Applications

Our Abpromise guarantee covers the use of ab94070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

PAI1 overexpression 293T lysate (whole cell) images

  • ab94070 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-PAI1 antibody (ab89581) at 1/500 dilution

    Lane 1 : PAI1 overexpression 293T lysate (whole cell) (ab94070)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for PAI1 overexpression 293T lysate (whole cell) (ab94070)

ab94070 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"