Anti-PAICS antibody - N-terminal (ab171381)

Overview

  • Product name
    Anti-PAICS antibody - N-terminal
    See all PAICS primary antibodies
  • Description
    Rabbit polyclonal to PAICS - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human PAICS aa 94-123 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: P22234

  • Positive control
    • K562 cell lysate, NIH-3T3 cell lysate, K562 cells, Human colon carcinoma tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab171381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 47 kDa.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Pathway
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
    Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
  • Sequence similarities
    In the N-terminal section; belongs to the SAICAR synthetase family.
    In the C-terminal section; belongs to the AIR carboxylase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADE2 antibody
    • ADE2H1 antibody
    • AIR carboxylase antibody
    • AIRC antibody
    • DKFZp781N1372 antibody
    • MGC1343 antibody
    • MGC5024 antibody
    • Multifunctional protein ADE2 antibody
    • Multifunctional protein ADE2H1 antibody
    • Paics antibody
    • PAIS antibody
    • Phosphoribosylaminoimidazole carboxylase antibody
    • Phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase antibody
    • PUR6_HUMAN antibody
    • SAICAR synthetase antibody
    see all

Images

  • Anti-PAICS antibody - N-terminal (ab171381) at 1/100 dilution + K562 cell lysate at 35 µg

    Predicted band size: 47 kDa

  • Anti-PAICS antibody - N-terminal (ab171381) at 1/100 dilution + Mouse NIH-3T3 cell lysate at 35 µg

    Predicted band size: 47 kDa

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human colon carcinoma labeling PAICS using ab171381 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) labeling PAICS using ab171381 at 1/10.  FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

References

ab171381 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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