1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route): step 1/1.
In the N-terminal section; belongs to the SAICAR synthetase family. In the C-terminal section; belongs to the AIR carboxylase family.
Immunohistochemistry analysis in formalin fixed and paraffin embedded Human colon carcinoma labeling PAICS using ab171381 at 1/10 dilution followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow Cytometry - Anti-PAICS antibody (ab171381)
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram) labeling PAICS using ab171381 at 1/10. FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.