Recombinant
RabMAb

Anti-PAIP1 antibody [EPR13258(B)] (ab175211)

Overview

  • Product name
    Anti-PAIP1 antibody [EPR13258(B)]
    See all PAIP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13258(B)] to PAIP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Human PAIP1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9H074

  • Positive control
    • HeLa, HepG2 and 293T cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13258(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab175211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
WB 1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function
      Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
    • Sequence similarities
      Contains 1 MIF4G domain.
    • Domain
      Only the PABPC1-interacting motif-1 (PAM1) stimulates translation initiation.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • MGC12360 antibody
      • OTTHUMP00000221601 antibody
      • OTTMUSP00000026880 antibody
      • PABC1 interacting protein 1 antibody
      • PABP interacting protein 1 antibody
      • PABP-interacting protein 1 antibody
      • PAIP 1 antibody
      • PAIP-1 antibody
      • Paip1 antibody
      • PAIP1_HUMAN antibody
      • Poly(A) binding protein interacting protein 1 antibody
      • Poly(A)-binding protein-interacting protein 1 antibody
      • Polyadenylate binding protein interacting protein 1 antibody
      • Polyadenylate-binding protein-interacting protein 1 antibody
      see all

    Images

    • All lanes : Anti-PAIP1 antibody [EPR13258(B)] (ab175211) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 54 kDa

    • Western blot analysis on immunoprecipitation pellet from (1) HeLa cells lysate or (2) 1X PBS (negative control) using ab175211, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab175211 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab175211.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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