Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment within Human PAK1 aa 151-544. The exact sequence is proprietary.
    Database link: Q13153

  • Positive control
    • Hepatoma tissue. A431 cells. HeLa, A431, BCL-1 and rat PC12 whole cell lysates/extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab183894 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 61 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localization
    Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Images

  • All lanes : Anti-PAK1 antibody (ab183894) at 1/5000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 61 kDa

  • Anti-PAK1 antibody (ab183894) at 1/1000 dilution + BCL-1 whole cell lysate/extract at 30 µg

    Predicted band size: 61 kDa

  • Anti-PAK1 antibody (ab183894) at 1/1000 dilution + rat PC12 whole cell lysate/extract at 30 µg

    Predicted band size: 61 kDa

  • Immunohistochemical analysis of paraffin-embedded human hepatoma labeling PAK1 using ab183894 at a 1/500 dilution.

  • Immunofluorescence analysis of A431 cells fixed in ice-cold methanol for 5 minutes, labeling PAK1 using ab183894 at a 1/500 dilution (green). Blue: Hoechst 33343 staining.

References

ab183894 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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