Overview

  • Product nameAnti-PAK1 antibody
    See all PAK1 primary antibodies
  • Description
    Goat polyclonal to PAK1
  • Specificityab77096 is not expected to react with PAK3.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Dog
  • Immunogen

    Peptide with sequence

    C-NTEKQKKKPKMSDE

    , corresponding to amino acids 238-251 from an internal region of human PAK1 protein (NP_002567.3)

  • Positive control
    • Human, mouse and rat brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesPeptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:4,000.
    WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 61 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated when activated by CDC42/p21 and RAC1.
    • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADRB2 antibody
      • Alpha PAK antibody
      • Alpha-PAK antibody
      • MGC130000 antibody
      • MGC130001 antibody
      • p21 activated kinase 1 antibody
      • p21 protein (Cdc42/Rac) activated kinase 1 antibody
      • p21-activated kinase 1 antibody
      • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
      • p65 PAK antibody
      • p65-PAK antibody
      • P68-PAK antibody
      • PAK alpha antibody
      • PAK-1 antibody
      • Pak1 antibody
      • PAK1_HUMAN antibody
      • Paka antibody
      • PAKalpha antibody
      • Protein kinase MUK2 antibody
      • Rac/p21-activated kinase antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog yeast antibody
      see all

    Anti-PAK1 antibody images

    • Anti-PAK1 antibody (ab77096) at 1 µg/ml + Rat brain lysate (in RIPA buffer) at 35 µg
      Developed using the ECL technique

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

    References for Anti-PAK1 antibody (ab77096)

    ab77096 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab77096.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"