Overview

  • Product nameAnti-PAK1 antibody
    See all PAK1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1
  • SpecificityDetects endogenous levels of total PAK1.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from Human PAK1 around the phosphorylation site of serine 199 (T-K-S-V-Y)

  • Positive control
    • K562 cells extracts; Human placenta tissue.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab79218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.

Target

  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 antibody images

  • Left panel: immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/50 ab79218; Right panel: same, after pre-incubation with the immunizing peptide.
  • All lanes : Anti-PAK1 antibody (ab79218) at 1/500 dilution

    Lane 1 : K562 cells extract
    Lane 2 : K562 cells extract with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa

References for Anti-PAK1 antibody (ab79218)

ab79218 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"