Overview

  • Product nameAnti-PAK1 antibody [EP795Y]
    See all PAK1 primary antibodies
  • Description
    Rabbit monoclonal [EP795Y] to PAK1
  • Tested applicationsSuitable for: Flow Cyt, IP, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in the N-terminus of human PAK1 protein.

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-PAK1 antibody (Alexa Fluor® 488) [EP795Y] (ab199088)

    Anti-PAK1 antibody (HRP) [EP795Y] (ab199311)

Properties

Applications

Our Abpromise guarantee covers the use of ab40852 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/50.
WB 1/500. Predicted molecular weight: 66 kDa.
IHC-P Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.

Target

  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 antibody [EP795Y] images



  • Predicted band size : 66 kDa
    Observed band size : 66 kDa
    Western blot analysis of anti- PAK1 (N-term) ab40852, dilution 1:500

    A: 10ug Hela

    B: 10ug A431

    C: 10ug Jurkat

    D: 10ug K562

    E: 10ug MCF7

    F: 10ug Daudi

    G: 10ug NIH 3T3

    H: 10ug PC12

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab40852.
  • Immunofluorescent staining of HeLa cells using ab40852.
  • Overlay histogram showing HeLa cells stained with ab40852 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab40852, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-PAK1 antibody [EP795Y] (ab40852)

This product has been referenced in:
  • Chiang CK  et al. The proteomic landscape of the suprachiasmatic nucleus clock reveals large-scale coordination of key biological processes. PLoS Genet 10:e1004695 (2014). WB ; Mouse . Read more (PubMed: 25330117) »
  • Maroto B  et al. P21-activated kinase is required for mitotic progression and regulates Plk1. Oncogene 27:4900-8 (2008). Human . Read more (PubMed: 18427546) »

See all 3 Publications for this product

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