Anti-PAK1 interacting protein 1 antibody (ab176813)

Overview

  • Product name
    Anti-PAK1 interacting protein 1 antibody
    See all PAK1 interacting protein 1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 interacting protein 1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Common marmoset, Orangutan
  • Immunogen

    Synthetic peptide within Human PAK1 interacting protein 1 aa 290-340. The exact sequence is proprietary. NP_060376.2
    Sequence:

    I NTNARLTCLG VWLDKVADMK ESLPPAAEPS PVSKEQSKIG KKEPGDTVHK


    Database link: Q9NWT1

  • Positive control
    • HeLa and 293T whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 99% Tris buffered saline, 0.1% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab176813 was affinity purified using an epitope specific to PAK1 interacting protein 1 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176813 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 44 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    PAK1 interacting protein 1 (PAK1IP1) negatively regulates the PAK1 kinase. PAK1 is a member of the PAK kinase family, which have been shown to play a positive role in the regulation of signaling pathways involving MAPK8 and RELA. PAK1 exists as an inactive homodimer, which is activated by binding of small GTPases such as CDC42 to an N terminal regulatory domain. PAK1IP1 also binds to the N terminus of PAK1, and inhibits the specific activation of PAK1 by CDC42.
  • Cellular localization
    Nuclear, nucleolus.
  • Database links
  • Alternative names
    • bA421M1.5 antibody
    • FLJ20624 antibody
    • hPIP 1 antibody
    • hPIP1 antibody
    • MAK 11 antibody
    • MAK11 antibody
    • p21 activated protein kinase interacting protein 1 antibody
    • PAK- AND PLC-INTERACTING PROTEIN 1 antibody
    • PAK/PLC interacting protein 1 antibody
    • PAK1 interacting protein antibody
    • PAK1IP1 antibody
    • PIP 1 antibody
    • PIP1 antibody
    • Putative PAK inhibitor Skb15 antibody
    • RP11 421M1.5 antibody
    • WD repeat containing protein 84 antibody
    • WDR 84 antibody
    • WDR84 antibody
    see all

Images

  • Detection of PAK1 interacting protein 1 by Western Blot of Immunprecipitate.
    ab176813 at 1µg/ml labeling PAK1 interacting protein 1 in HeLa whole cell lysate immunoprecipitated using ab176813 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.

  • All lanes : Anti-PAK1 interacting protein 1 antibody (ab176813) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 44 kDa


    Exposure time : 3 minutes

References

ab176813 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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