The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
RelevanceIn mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 is mostly brain specific, while PAK2 is expressed ubiquitously.
The family of serine/threonine p21 activated kinases (PAK) are Rho GTPases effectors for cytoskeleton reorganization and nuclear signaling. They are targets for the small GTP binding proteins, CDC42 and RAC1, and have been implicated in a wide range of biological activities.