Anti-PAK1 + PAK2 + PAK3 antibody (ab196834)
Key features and details
- Rabbit polyclonal to PAK1 + PAK2 + PAK3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PAK1 + PAK2 + PAK3 antibody
See all PAK1 + PAK2 + PAK3 primary antibodies -
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Human PAK1 + PAK2 + PAK3. (Synthesized non-phosphopeptide derived from Human PAK1 + PAK2 + PAK3 around the phosphorylation site of threonine 423/402/421 (R-S-T(p)-M-V). [UniProt Q13153, O75914 andQ13177]).
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Positive control
- Human brain tissue; NIH/3T3 and 293 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS without Mg2+ and Ca2+. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab196834 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab196834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/3000. Predicted molecular weight: 61, 58, 62 kDa.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/3000. Predicted molecular weight: 61, 58, 62 kDa. |
Target
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Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death. -
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation -
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 18481 Mouse
- Entrez Gene: 224105 Mouse
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
see all -
Alternative names
- Alpha PAK antibody
- Beta PAK antibody
- bPAK antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling PAK1 + PAK2 + PAK3 with ab196834 at 1/50 dilution (left) and synthesized peptide (right).
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All lanes : Anti-PAK1 + PAK2 + PAK3 antibody (ab196834) at 1/500 dilution
Lane 1 : NIH/3T3 cell extract
Lane 2 : NIH/3T3 cell extract with synthesized peptide
Predicted band size: 61, 58, 62 kDa -
All lanes : Anti-PAK1 + PAK2 + PAK3 antibody (ab196834) at 1/500 dilution
Lane 1 : 293 cell extract with synthesized peptide
Lane 2 : 293 cell extract
Predicted band size: 61, 58, 62 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab196834 has been referenced in 1 publication.
- Lee SH et al. Haploinsufficiency of Cyfip2 Causes Lithium-Responsive Prefrontal Dysfunction. Ann Neurol 88:526-543 (2020). PubMed: 32562430