Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PAK1 + PAK2 + PAK3 (phospho S144 + S141 + S139) antibody
See all PAK1 + PAK2 + PAK3 products (9) ...
Description
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho S144 + S141 + S139)
Specificity
This antibody detects endogenous levels of PAK1 + PAK2 + PAK3 only when phosphorylated at serine 144 + 141 + 139.
Tested applications
ELISA, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthesized phosphopeptide derived from human PAK1 + PAK2 + PAK3 around the phosphorylation site of serine 144 + 141 + 139
Positive control
Human brain tissue sections
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> Structures >> Focal Adhesions
Cell Biology >> Apoptosis >> Intracellular >> Kinases
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK1 + PAK2 + PAK3 (phospho S144/141/139) antibody (ab63513)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000.ELISA: 1/10000.
IHC-P: 1/50 - 1/100.
Target
Relevance
In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localization
Cytoplasm. Recruited to focal adhesions upon activation
Alternative names
- Alpha PAK antibody
- AlphaPAK antibody
- Beta PAK antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 5062 Human
- Entrez Gene: 5063 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 224105 Mouse
- Entrez Gene: 18481 Mouse
- Entrez Gene: 29431 Rat
- Entrez Gene: 29432 Rat
see all
Anti-PAK1 + PAK2 + PAK3 (phospho S144 + S141 + S139) antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PAK1 + PAK2 + PAK3 (phospho S144/141/139) antibody (ab63513)
ab63513 at a 1:50 dilution, staining PAK1 + PAK2 + PAK3 in human brain tissueLeft image un-treated.Right image treated with phosphopeptide.
References for Anti-PAK1 + PAK2 + PAK3 (phospho S144 + S141 + S139) antibody (ab63513)
ab63513 has not yet been referenced specifically in any publications.
Publishing research using ab63513? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"