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Product name Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody See all PAK1 + PAK2 + PAK3 primary antibodies
Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)
Specificity ab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).
Tested applications Suitable for:
WB more details
Mouse, Rat, Human
Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.
General notes Note: Thr402 in PAK2 corresponds to Thr423 in human PAK1.
Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES. pH 7.5
Concentration information loading...
Purity Immunogen affinity purified
Purification notes ab30577 was purified by sequential chromatography on phospho and dephosphopeptide affinity columns.
Primary antibody notes Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).
Relevance In mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localization Cytoplasm. Recruited to focal adhesions upon activation
Alpha PAK antibody
Beta PAK antibody
Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody images
Western blot - PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution Lane 1 : untreated rat hippocampal lysate Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control) Predicted band size : 60 kDa Observed band size : 68 kDa ( why is the actual band size different from the predicted?)
References for Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"