Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)

Overview

  • Product nameAnti-PAK1 + PAK2 + PAK3 (phospho T402) antibody
    See all PAK1 + PAK2 + PAK3 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T402)
  • Specificityab30577 recognises PAK1 + PAK2 + PAK3 (phospho T402).
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic Phosphopeptide corresponding to amino acid residues surrounding the phospho Thr402 of rat p21 Activated Kinase 2 (PAK2). The peptide sequence used is identical in PAK1, 2 and 3.

  • Positive control
    • Rat hippocampal lysate
  • General notesNote: Thr402 in PAK2 corresponds to Thr423 in human PAK1.


    Autophosphorylation of Thr402 in the protein has been found to be essential for activation of PAK.

Properties

Applications

Our Abpromise guarantee covers the use of ab30577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 60 kDa).

Target

Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody images

  • All lanes : Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577) at 1/1000 dilution

    Lane 1 : untreated rat hippocampal lysate
    Lane 2 : rat hippocampal lysate, pretreated with 1200 units lambda Ptase for 30 min (negative control)


    Predicted band size : 60 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-PAK1 + PAK2 + PAK3 (phospho T402) antibody (ab30577)

ab30577 has not yet been referenced specifically in any publications.

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