Overview

  • Product nameAnti-PAK1 + PAK2 + PAK3 (phospho T423) antibody
    See all PAK1 + PAK2 + PAK3 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 + PAK2 + PAK3 (phospho T423)
  • SpecificityThis phospho specific polyclonal antibody is specific for phosphorylated Threonine 423 of Human PAK 1 + 2 + 3. Reactivity with non-phosphorylated PAK 1 + 2 + 3 is less than 1% by ELISA.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: EQSKRSTpMVGTPY conjugated to KLH, corresponding to amino acids 413-433 of Human PAK 1, 408-428 of human PAK 2 and 411-431 of human PAK 3.

  • General notes


    P21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide.
  • Primary antibody notesP21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases. The three most common isoforms are PAK 1, PAK 2, and PAK 3 (also known as alpha PAK, gamma PAK, and beta PAK, respectively). These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspase mediated proteolytic cleavage. Autophosphorylation of threonine 423 (threonine 402 for PAK 2 and threonine 421 for PAK 3), catalyzed by Cdc42, is required for activation of PAK.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2477 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/4000 - 1/20000.
WB 1/500 - 1/2000. Detects a band of approximately 65 kDa.

Target

  • RelevanceIn mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
  • Cellular localizationCytoplasm. Recruited to focal adhesions upon activation
  • Database links
  • Alternative names
    • Alpha PAK antibody
    • Beta PAK antibody
    • bPAK antibody
    • CDKN1A antibody
    • Gamma PAK antibody
    • hPAK3 antibody
    • MRX30 antibody
    • MRX47 antibody
    • Oligophrenin 3 antibody
    • OPHN3 antibody
    • P21 (CDKN1A) activated kinase 2 antibody
    • P21 (CDKN1A) activated kinase 3 antibody
    • p21 activated kinase 1 antibody
    • p21 activated kinase 2 antibody
    • p21 activated kinase 3 antibody
    • P21 protein (Cdc42/Rac) activated kinase 1 antibody
    • P21 protein (Cdc42/Rac) activated kinase 2 antibody
    • P21 protein (Cdc42/Rac) activated kinase 3 antibody
    • P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog, yeast) antibody
    • P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • P58 antibody
    • P65 PAK antibody
    • PAK 2 antibody
    • PAK 3 antibody
    • PAK1 antibody
    • PAK2 antibody
    • PAK3 antibody
    • PAK3beta antibody
    • PAK65 antibody
    • PAKalpha antibody
    • PAKgamma antibody
    • S6/H4 kinase antibody
    • Serine/threonine protein kinase PAK 1 antibody
    • Serine/threonine protein kinase PAK 2 antibody
    • Serine/threonine protein kinase PAK 3 antibody
    • Serine/threonine protein kinase PAK1 antibody
    • Serine/threonine protein kinase PAK2 antibody
    • Serine/threonine protein kinase PAK3 antibody
    • STE20 homolog, yeast antibody
    see all

Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody images

  • Western blot probing with PAK1 antibody (ab2477). The major band is at 65 kDa and weaker bands can be detected at 90 and 100 kDa. This picture was kindly submitted by Sue Thomas as part of her review.

References for Anti-PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab2477)

This product has been referenced in:
  • Gadepalli R  et al. Novel Role for p21-activated Kinase 2 in Thrombin-induced Monocyte Migration. J Biol Chem 288:30815-31 (2013). WB . Read more (PubMed: 24025335) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"