SpecificityDetects endogenous levels of PAK1, PAK2 and PAK3 only when phosphorylated at threonine 423, threonine 402 and threonine 421 respectively (Human: Thr423/402/421; Mouse: Thr423/402/421; Rat: Thr422/402/421).
Purification notesPurified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
RelevanceIn mammals, there are several identified isoforms of p21 Activated Protein Kinases or PAKs: PAK1 and PAK3 are mostly brain specific, while PAK2 is expressed ubiquitously. Mutations of the gene coding for PAK3 are associated with X linked mental retardation and PAK3 is a key regulator of synapse formation and plasticity in the hippocampus. PAK3 is thought to play a key role in regulation of cell shape and motility as well as cell death.
Cellular localizationCytoplasm. Recruited to focal adhesions upon activation
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using PAK1 + PAK2 + PAK3 (phospho T423) antibody (ab62155) at 1/50 - 1/100 dilution, in the presence (right panel) and absence (left panel) of immunising phosphopeptide.