![Western blot - PAK1 antibody [EP656Y] (ab40795) image](#ab40795wb.jpg)
![Immunohistochemistry (Paraffin-embedded sections) - PAK1 (phospho S144) antibody [EP656Y] (ab40795) image](#ab40795IHCP.jpg)
![Immunocytochemistry/ Immunofluorescence - PAK1 (phospho S144) antibody [EP656Y] (ab40795) image](#ab40795IF.jpg)
Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Related products
- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Overview
Product name
Anti-PAK1 (phospho S144) antibody [EP656Y]
See all PAK1 products (19) ...
Description
Rabbit monoclonal [EP656Y] to PAK1 (phospho S144)
Specificity
ab40795 recognises p21-activated kinase 1 (PAK1).
Tested applications
WB, IHC-P, ICC, IP, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide corresponding to a sequence containing phosphoserine 144 of the human PAK1 protein.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Monoclonal
Clone number
EP656Y
Isotype
IgG
Research areas
Cancer >> Signal transduction >> Protein phosphorylation >> Serine/threonine kinases >> Other
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Cell Biology >> Apoptosis >> Intracellular >> Kinases
Applications
Show applications key
Our Abpromise guarantee covers the use of ab40795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
ICC
IP
Application notes
ICC: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/10000 - 1/50000. Detects a band of approximately 66 kDa (predicted molecular weight: 65 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Target information above from: UniProt accessionQ13153
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
Database links
- Entrez Gene: 5058 Human
- Entrez Gene: 18479 Mouse
- Entrez Gene: 29431 Rat
- Omim: 602590 Human
- SwissProt: Q13153 Human
- SwissProt: O88643 Mouse
- SwissProt: P35465 Rat
- Unigene: 435714 Human
see all
Anti-PAK1 (phospho S144) antibody [EP656Y] images:
Western blot - PAK1 antibody [EP656Y] (ab40795)
![Western blot - PAK1 antibody [EP656Y] (ab40795)](/ps/datasheet/Images/40/ab40795/ab40795wb.jpg)
Anti-PAK1 (phospho S144) antibody [EP656Y] (ab40795) at 1/50000 dilution + HeLa cell lysate
Predicted band size : 65 kDa
Observed band size : 65 kDa
Immunohistochemistry (Paraffin-embedded sections) - PAK1 (phospho S144) antibody [EP656Y] (ab40795)
![Immunohistochemistry (Paraffin-embedded sections) - PAK1 (phospho S144) antibody [EP656Y] (ab40795)](/ps/datasheet/Images/40/ab40795/ab40795IHCP.jpg)
Immunohistochemical analysis of paraffin-embedded human colon cancer using ab40795.
Immunocytochemistry/ Immunofluorescence - PAK1 (phospho S144) antibody [EP656Y] (ab40795)
![Immunocytochemistry/ Immunofluorescence - PAK1 (phospho S144) antibody [EP656Y] (ab40795)](/ps/datasheet/Images/40/ab40795/ab40795IF.jpg)
Immunofluorescent staining of HeLa cells using ab40795.
Western blot - PAK1 (phospho S144) antibody [EP656Y] (ab40795)
![Western blot - PAK1 (phospho S144) antibody [EP656Y] (ab40795)](/ps/datasheet/images/40/ab40795/PAK1-Primary-antibodies-ab40795-2.jpg)
All lanes : Anti-PAK1 (phospho S144) antibody [EP656Y] (ab40795)
Lane 1 : HeLa Cell lysate
Lane 2 : HeLa Cell lysate treated with lambda phosphatase
Predicted band size : 65 kDa
Observed band size : 65 kDa
References for Anti-PAK1 (phospho S144) antibody [EP656Y] (ab40795)
This product has been referenced in:
- Long W et al. SRC-3Delta4 mediates the interaction of EGFR with FAK to promote cell migration. Mol Cell 37:321-32 (2010). WB, ICC/IF; Mouse.Read more (PubMed: 20159552) »
- Kim SH et al. Down-regulation of p21-activated kinase 1 by progestin and its increased expression in the eutopic endometrium of women with endometriosis. Hum Reprod 24:1133-41 (2009).Read more (PubMed: 19168872) »
See all 5 publications for this product
Publishing research using ab40795? Please let us know so that we can cite the reference in this datasheet
![PAK1 antibody [EP656Y] for ICC/IF in Mouse (40795)](/ps/Reviews/Images/ab6973_27716.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"