Human Predicted to work with:
Synthetic peptide within Human PAK1 (phospho S199) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of Serine 199 (T-K-S(p)-V-I). Database link: Q13153
Lysate of starved LOVO cells and Human placenta tissue.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Autophosphorylated when activated by CDC42/p21 and RAC1.
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.