Overview

  • Product name
    Anti-PAK1 (phospho S199) antibody
    See all PAK1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 (phospho S199)
  • Host species
    Rabbit
  • Specificity
    ab192814 detects endogenous levels of PAK1 only when phosphorylated at serine 199.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human PAK1 (phospho S199) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. Peptide sequence around phosphorylation site of Serine 199 (T-K-S(p)-V-I).
    Database link: Q13153

  • Positive control
    • Lysate of starved LOVO cells and Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab192814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localization
    Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Images

  • All lanes : Anti-PAK1 (phospho S199) antibody (ab192814) at 1/500 dilution

    Lane 1 : Lysate of starved LOVO cells
    Lane 2 : Lysate of starved LOVO cells with antigen-specific peptide

    Predicted band size: 61 kDa

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling PAK1 (phospho S199) using ab192814 at a 1/50 dilution, the right panel was treated with blocking peptide.

References

This product has been referenced in:
  • Feng Y  et al. Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress. Front Physiol 7:176 (2016). Read more (PubMed: 27303303) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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