Recombinant Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP655Y] to PAK1 (phospho S20)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PAK1 (phospho S20) antibody [EP655Y]
See all PAK1 primary antibodies -
Description
Rabbit monoclonal [EP655Y] to PAK1 (phospho S20) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human PAK1 (phospho S20). Numbering corresponds to mature PAK1 without Initiator methionine.
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Positive control
- HeLa cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.31% Sodium citrate, 0.175% Sodium chloride, 0.0172% EDTA, 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP655Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab51244 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
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Notes |
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WB
1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). |
Target
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Function
The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 CRIB domain.
Contains 1 protein kinase domain. -
Post-translational
modificationsAutophosphorylated when activated by CDC42/p21 and RAC1. -
Cellular localization
Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation. - Information by UniProt
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Database links
- Entrez Gene: 5058 Human
- Omim: 602590 Human
- SwissProt: Q13153 Human
- Unigene: 435714 Human
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Alternative names
- Alpha PAK antibody
- Alpha-PAK antibody
- MGC130000 antibody
see all
Images
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All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) Whole cell lysates
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes Whole cell lysates
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100mM calyculin A for 30 minutes. Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesWe are unable to define the extra bands
Blocking buffer: 5% NFDM/TBST
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab51244 has not yet been referenced specifically in any publications.