Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244)

Overview

  • Product nameAnti-PAK1 (phospho S20) antibody [EP655Y]
    See all PAK1 primary antibodies
  • Description
    Rabbit monoclonal [EP655Y] to PAK1 (phospho S20)
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK1.

  • Positive control
    • HeLa cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab51244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesIP: 1/50.
    WB: 1/2000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).

    Is unsuitable for Flow Cyt or IHC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated when activated by CDC42/p21 and RAC1.
    • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
    • Information by UniProt
    • Database links
    • Alternative names
      • ADRB2 antibody
      • Alpha PAK antibody
      • Alpha-PAK antibody
      • MGC130000 antibody
      • MGC130001 antibody
      • p21 activated kinase 1 antibody
      • p21 protein (Cdc42/Rac) activated kinase 1 antibody
      • p21-activated kinase 1 antibody
      • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
      • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
      • p65 PAK antibody
      • p65-PAK antibody
      • P68-PAK antibody
      • PAK alpha antibody
      • PAK-1 antibody
      • Pak1 antibody
      • PAK1_HUMAN antibody
      • Paka antibody
      • PAKalpha antibody
      • Protein kinase MUK2 antibody
      • Rac/p21-activated kinase antibody
      • Serine/threonine-protein kinase PAK 1 antibody
      • STE20 homolog yeast antibody
      see all

    Anti-PAK1 (phospho S20) antibody [EP655Y] images

    • All lanes : Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244) at 1/2000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate, treated with AP

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 61 kDa
      Observed band size : 61 kDa

    References for Anti-PAK1 (phospho S20) antibody [EP655Y] (ab51244)

    ab51244 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"