Anti-PAK1 (phospho S204) antibody (ab79503)

Overview

  • Product nameAnti-PAK1 (phospho S204) antibody
    See all PAK1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 (phospho S204)
  • Specificityab79503 detects endogenous levels of PAK1 only when phosphorylated at serine 204.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of serine 204 (TRSPVI)

  • Positive control
    • IHC: human brain tissue WB: extracts from 3T3 cells (treated with UV for 15mins)

Properties

Applications

Our Abpromise guarantee covers the use of ab79503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

Target

  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 (phospho S204) antibody images

  • All lanes : Anti-PAK1 (phospho S204) antibody (ab79503) at 1/1000 dilution

    Lane 1 : extracts from 3T3 cells (treated with UV for 15 mins)
    Lane 2 : extracts from 3T3 cells (treated with UV for 15 mins) with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 61 kDa
    Observed band size : 61 kDa
  • Immunohistochemistry analysis of paraffin-embedded human brain tissue, stained with ab79503, at 1/100 dilution, in the absence and presence of the immunising peptide

References for Anti-PAK1 (phospho S204) antibody (ab79503)

ab79503 has not yet been referenced specifically in any publications.

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