Anti-PAK1 (phospho T212) antibody (ab58540)

Overview

  • Product nameAnti-PAK1 (phospho T212) antibody
    See all PAK1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 (phospho T212)
  • Specificityab58540 detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).

  • Positive control
    • Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab58540 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000.
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 (phospho T212) antibody images

  • ab58540 at 1/50 dilution staining PAK1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

References for Anti-PAK1 (phospho T212) antibody (ab58540)

ab58540 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (normal tissue array (including breast/colon/spleen)
Specification normal tissue array (including breast/colon/spleen
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization No
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 10%
Username

Abcam user community

Verified customer

Submitted May 19 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"