Overview

  • Product name
    Anti-PAK1 (phospho T212) antibody
    See all PAK1 primary antibodies
  • Description
    Rabbit polyclonal to PAK1 (phospho T212)
  • Specificity
    ab75599 detects endogenous levels of PAK1 only when phosphorylated at threonine 212.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 212 (P-V-TP-P-T).

  • Positive control
    • Extracts from 293 cells treated with forskolin (40µM, 30min). Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab75599 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21 and RAC1.
  • Cellular localization
    Cytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADRB2 antibody
    • Alpha PAK antibody
    • Alpha-PAK antibody
    • MGC130000 antibody
    • MGC130001 antibody
    • p21 activated kinase 1 antibody
    • p21 protein (Cdc42/Rac) activated kinase 1 antibody
    • p21-activated kinase 1 antibody
    • p21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody
    • p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) antibody
    • p65 PAK antibody
    • p65-PAK antibody
    • P68-PAK antibody
    • PAK alpha antibody
    • PAK-1 antibody
    • Pak1 antibody
    • PAK1_HUMAN antibody
    • Paka antibody
    • PAKalpha antibody
    • Protein kinase MUK2 antibody
    • Rac/p21-activated kinase antibody
    • Serine/threonine-protein kinase PAK 1 antibody
    • STE20 homolog yeast antibody
    see all

Anti-PAK1 (phospho T212) antibody images

  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
  • All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution

    Lane 1 : Extracts from 293 cells (untreated)
    Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),


    Predicted band size : 61 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab75599 at 1/100 dilution staining PAK1 (phospho T212) in Hela cells by ICC/IF (MeOH fixation).

References for Anti-PAK1 (phospho T212) antibody (ab75599)

This product has been referenced in:
  • Wang Z  et al. Clinicopathological signature of p21-activated kinase 1 in prostate cancer and its regulation of proliferation and autophagy via the mTOR signaling pathway. Oncotarget 8:22563-22580 (2017). IHC-P ; Human . Read more (PubMed: 28186966) »
  • Feng Y  et al. Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress. Front Physiol 7:176 (2016). Read more (PubMed: 27303303) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HCT116)
Loading amount
50 µg
Specification
HCT116
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 08 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up