Purification notesab75599 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using a non-phosphopeptide corresponding to the phosphorylation site.
FunctionThe activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated when activated by CDC42/p21 and RAC1.
Cellular localizationCytoplasm. Cell junction > focal adhesion. Recruited to focal adhesions upon activation.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75599, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
Western blot - PAK1 (phospho T212) antibody (ab75599)
All lanes : Anti-PAK1 (phospho T212) antibody (ab75599) at 1/500 dilution
Lane 1 : Extracts from 293 cells (untreated) Lane 2 : Extracts from 293 cells treated with forskolin (40µM, 30min),