• Product nameAnti-PAK2 antibody
    See all PAK2 primary antibodies
  • Description
    Rabbit polyclonal to PAK2
  • Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic 14 amino acid peptide from near the carboxy terminus of human PAK2.

  • Positive control
    • Jurkat lysate.


Associated products


Our Abpromise guarantee covers the use of ab45426 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).


  • FunctionThe activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
  • Tissue specificityUbiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
    During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
    Ubiquitinated, leading to its proteasomal degradation.
    PAK-2p34 is myristoylated.
  • Cellular localizationCytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-t-PAK2 antibody
    • CB422 antibody
    • EC antibody
    • Gamma PAK antibody
    • Gamma-PAK antibody
    • hPAK65 antibody
    • Kinase antibody
    • p21 (CDKN1A) activated kinase 2 antibody
    • p21 (CDKN1A)-activated kinase 2a antibody
    • p21 activated kinase 2 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
    • p21 protein Cdc42 Rac activated kinase 2 antibody
    • p21-activated kinase 2 antibody
    • p21-activated kinase, 65-KD antibody
    • p21-activated protein kinase I antibody
    • p21CDKN1A activated kinase 2 antibody
    • p27 antibody
    • p34 antibody
    • p58 antibody
    • p65PAK antibody
    • PAK 2 antibody
    • PAK-2 antibody
    • PAK-2p34 antibody
    • Pak2 antibody
    • PAK2_HUMAN antibody
    • PAK65 antibody
    • PAKgamma antibody
    • S6 H4 kinase antibody
    • S6/H4 kinase antibody
    • Serine threonine protein kinase PAK 2 antibody
    • Serine/threonine protein kinase PAK 2 antibody
    see all

Anti-PAK2 antibody images

  • Lane 1 : Anti-PAK2 antibody (ab45426) at 0.5 µg/ml
    Lane 2 : Anti-PAK2 antibody (ab45426) at 1 µg/ml

    Lane 1 : Jurkat lysate
    Lane 2 : Jurkat lysate

    Lysates/proteins at 15 µg per lane.

    anti-rabbit HRP

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
    Additional bands at : 49 kDa. We are unsure as to the identity of these extra bands.
  • ab45426 at 10µg/ml staining PAK2 in mouse spleen tissue by IHC

  • Immunofluorescence of PAK2 in Mouse Spleen cells using ab45426 at 20 ug/ml.

References for Anti-PAK2 antibody (ab45426)

This product has been referenced in:
  • Gao C  et al. Activation of P21-activated protein kinase 2 is an independent prognostic predictor for patients with gastric cancer. Diagn Pathol 9:55 (2014). IHC-P ; Human . Read more (PubMed: 24621074) »

See 1 Publication for this product

Product Wall

Alignment analysis within the PAK family members and the immunogen for catalog number 3885 show 100% conservation with PAK1 and 13/14 residues that are identical with PAK3. Therefore, it is quite likely that the antibody will cross-react with all 3 fam...

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