• Product nameAnti-PAK2 (phospho S20) antibody
    See all PAK2 primary antibodies
  • Description
    Rabbit polyclonal to PAK2 (phospho S20)
  • Specificityab59359 detects endogenous levels of PAK2 only when phosphorylated at serine 20.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human PAK2 around the phosphorylation site of serine 20 (MSSPTI).

  • Positive control
    • 293 whole cell lysate (WB) Human colon carcinoma tissue (IHC)
  • General notesStore at -20°C for 1 year


Associated products


Our Abpromise guarantee covers the use of ab59359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 58 kDa.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.


  • FunctionThe activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
  • Tissue specificityUbiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
    During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
    Ubiquitinated, leading to its proteasomal degradation.
    PAK-2p34 is myristoylated.
  • Cellular localizationCytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • C-t-PAK2 antibody
    • CB422 antibody
    • EC antibody
    • Gamma PAK antibody
    • Gamma-PAK antibody
    • hPAK65 antibody
    • Kinase antibody
    • p21 (CDKN1A) activated kinase 2 antibody
    • p21 (CDKN1A)-activated kinase 2a antibody
    • p21 activated kinase 2 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
    • p21 protein Cdc42 Rac activated kinase 2 antibody
    • p21-activated kinase 2 antibody
    • p21-activated kinase, 65-KD antibody
    • p21-activated protein kinase I antibody
    • p21CDKN1A activated kinase 2 antibody
    • p27 antibody
    • p34 antibody
    • p58 antibody
    • p65PAK antibody
    • PAK 2 antibody
    • PAK-2 antibody
    • PAK-2p34 antibody
    • Pak2 antibody
    • PAK2_HUMAN antibody
    • PAK65 antibody
    • PAKgamma antibody
    • S6 H4 kinase antibody
    • S6/H4 kinase antibody
    • Serine threonine protein kinase PAK 2 antibody
    • Serine/threonine protein kinase PAK 2 antibody
    see all

Anti-PAK2 (phospho S20) antibody images

  • All lanes : Anti-PAK2 (phospho S20) antibody (ab59359) at 1/500 dilution

    Lane 1 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30min) with no immunizing peptide
    Lane 2 : Extracts from 293 cells, treated with Sorbitol (0.4M, 30mins) with immunizing peptide

    Observed band size : 58 kDa (why is the actual band size different from the predicted?)
  • ab59359, at a 1/50 dilution, staining human PAK2 (phospho S20) in colon carcinoma, using Immunohistochemistry, Paraffin embedded tissue, in the absence of the immunizing peptide (left image) and in the presence of the immunizing peptide (right image).

References for Anti-PAK2 (phospho S20) antibody (ab59359)

This product has been referenced in:
  • Gao C  et al. Activation of P21-activated protein kinase 2 is an independent prognostic predictor for patients with gastric cancer. Diagn Pathol 9:55 (2014). IHC-P ; Human . Read more (PubMed: 24621074) »

See 1 Publication for this product

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