Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)


  • Product name
    Anti-PAK2 (phospho S20) antibody [EPR658(2)]
    See all PAK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20)
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK2

  • Positive control
    • HeLa cell lysate treated with calyculin A
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab76419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      The activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
    • Tissue specificity
      Ubiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
      During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
      Ubiquitinated, leading to its proteasomal degradation.
      PAK-2p34 is myristoylated.
    • Cellular localization
      Cytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-t-PAK2 antibody
      • CB422 antibody
      • EC antibody
      • Gamma PAK antibody
      • Gamma-PAK antibody
      • hPAK65 antibody
      • Kinase antibody
      • p21 (CDKN1A) activated kinase 2 antibody
      • p21 (CDKN1A)-activated kinase 2a antibody
      • p21 activated kinase 2 antibody
      • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
      • p21 protein Cdc42 Rac activated kinase 2 antibody
      • p21-activated kinase 2 antibody
      • p21-activated kinase, 65-KD antibody
      • p21-activated protein kinase I antibody
      • p21CDKN1A activated kinase 2 antibody
      • p27 antibody
      • p34 antibody
      • p58 antibody
      • p65PAK antibody
      • PAK 2 antibody
      • PAK-2 antibody
      • PAK-2p34 antibody
      • Pak2 antibody
      • PAK2_HUMAN antibody
      • PAK65 antibody
      • PAKgamma antibody
      • S6 H4 kinase antibody
      • S6/H4 kinase antibody
      • Serine threonine protein kinase PAK 2 antibody
      • Serine/threonine protein kinase PAK 2 antibody
      see all


    • All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa


    ab76419 has not yet been referenced specifically in any publications.

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