Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)


  • Product nameAnti-PAK2 (phospho S20) antibody [EPR658(2)]
    See all PAK2 primary antibodies
  • Description
    Rabbit monoclonal [EPR658(2)] to PAK2 (phospho S20)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Serine 20 of human PAK2

  • Positive control
    • HeLa cell lysate treated with calyculin A
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionThe activated kinase acts on a variety of targets. Phosphorylates ribosomal protein S6, histone H4 and myelin basic protein. Full length PAK 2 stimulates cell survival and cell growth. The process is, at least in part, mediated by phosphorylation and inhibition of pro-apoptotic BAD. Caspase-activated PAK-2p34 is involved in cell death response, probably involving the JNK signaling pathway. Cleaved PAK-2p34 seems to have a higher activity than the CDC42-activated form.
    • Tissue specificityUbiquitously expressed. Higher levels seen in skeletal muscle, ovary, thymus and spleen.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      Full length PAK 2 is autophosphorylated when activated by CDC42/p21. Following cleavage, both peptides, PAK-2p27 and PAK-2p34, become highly autophosphorylated, with PAK-2p27 being phosphorylated on serine and PAK-2p34 on threonine residues, respectively. Autophosphorylation of PAK-2p27 can occur in the absence of any effectors and is dependent on phosphorylation of Thr-402, because PAK-2p27 is acting as an exogenous substrate.
      During apoptosis proteolytically cleaved by caspase-3 or caspase-3-like proteases to yield active PAK-2p34.
      Ubiquitinated, leading to its proteasomal degradation.
      PAK-2p34 is myristoylated.
    • Cellular localizationCytoplasm and Nucleus. Cytoplasm > perinuclear region. Membrane. Interaction with ARHGAP10 probably changes PAK-2p34 location to cytoplasmic perinuclear region. Myristoylation changes PAK-2p34 location to the membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • C-t-PAK2 antibody
      • CB422 antibody
      • EC antibody
      • Gamma PAK antibody
      • Gamma-PAK antibody
      • hPAK65 antibody
      • Kinase antibody
      • p21 (CDKN1A) activated kinase 2 antibody
      • p21 (CDKN1A)-activated kinase 2a antibody
      • p21 activated kinase 2 antibody
      • p21 protein (Cdc42/Rac)-activated kinase 2 antibody
      • p21 protein Cdc42 Rac activated kinase 2 antibody
      • p21-activated kinase 2 antibody
      • p21-activated kinase, 65-KD antibody
      • p21-activated protein kinase I antibody
      • p21CDKN1A activated kinase 2 antibody
      • p27 antibody
      • p34 antibody
      • p58 antibody
      • p65PAK antibody
      • PAK 2 antibody
      • PAK-2 antibody
      • PAK-2p34 antibody
      • Pak2 antibody
      • PAK2_HUMAN antibody
      • PAK65 antibody
      • PAKgamma antibody
      • S6 H4 kinase antibody
      • S6/H4 kinase antibody
      • Serine threonine protein kinase PAK 2 antibody
      • Serine/threonine protein kinase PAK 2 antibody
      see all

    Anti-PAK2 (phospho S20) antibody [EPR658(2)] images

    • All lanes : Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419) at 1/20000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HeLa cell lysate treated with calyculin A

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 58 kDa
      Observed band size : 58 kDa

    References for Anti-PAK2 (phospho S20) antibody [EPR658(2)] (ab76419)

    ab76419 has not yet been referenced specifically in any publications.

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