Overview

  • Product nameAnti-PAK3 (phospho S154) antibody
    See all PAK3 primary antibodies
  • Description
    Rabbit polyclonal to PAK3 (phospho S154)
  • Specificityab80181 detects endogenous levels of PAK3 only when phosphorylated at serine 154.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic phosphopeptide derived from human PAK3 around the phosphorylation site of serine 154 (Y-M-SP-F-T).

  • Positive control
    • Human brain tissue; rat heart cell extracts. IF/ICC: PC12 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab80181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 62 kDa.
ELISA 1/20000.
IHC-P 1/50 - 1/100.

Target

  • RelevancePAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities. PAK3 forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1 proteins which then catalyzes a variety of targets. It may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. A point mutation in this gene has been linked to nonsyndromic X-linked mental retardation.
  • Cellular localizationCytoplasmic
  • Database links
  • Alternative names
    • Beta PAK antibody
    • bPAK antibody
    • CDKN1A antibody
    • hPAK3 antibody
    • Mental retardation X linked 30 antibody
    • MRX30 antibody
    • MRX47 antibody
    • Oligophrenin 3 antibody
    • OPHN3 antibody
    • p21 (CDKN1A) activated kinase 3 antibody
    • p21 activated kinase 3 antibody
    • p21 CDKN1A activated kinase 3 antibody
    • P21 protein (Cdc42/Rac) activated kinase 3 antibody
    • PAK-3 antibody
    • PAK3 p21 protein (Cdc42/Rac)-activated kinase 3 antibody
    • PAK3beta antibody
    • Pak65alpha antibody
    • Pak65beta antibody
    • Serine threonine protein kinase PAK 3 antibody
    • Stk4 antibody
    see all

Anti-PAK3 (phospho S154) antibody images

  • ICC/IF image of ab80181 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80181, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab80181, at a 1/50 dilution, staining PAK3 in paraffin embedded human brain tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunizing phospho peptide.
  • All lanes : Anti-PAK3 (phospho S154) antibody (ab80181) at 1/500 dilution

    Lane 1 : rat heart cell extracts
    Lane 2 : rat heart cell extracts with immunizing phospho peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 62 kDa
    Observed band size : 62 kDa

References for Anti-PAK3 (phospho S154) antibody (ab80181)

This product has been referenced in:
  • van Rahden VA  et al. The 5-phosphatase OCRL mediates retrograde transport of the mannose 6-phosphate receptor by regulating a Rac1-cofilin signalling module. Hum Mol Genet 21:5019-38 (2012). WB ; Human . Read more (PubMed: 22907655) »

See 1 Publication for this product

Product Wall

Thank you fo ryour reply.

Reviewing the image and details provided, I can suggest your results are as we woudl expect them to be for detection of PAK3 (phospho S154). The blot shows a good clear band at the expected size and the rest of the ...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for WB ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"