Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK4
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Dog, Pig, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide representing a portion of human p21(CDKN1A) activated kinase 4 encoded within exon 4 (Locuslink ID 10298).

  • Positive control
    • Whole cell lysate of Hela or Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab17974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes

    IP: 1 to 4 mcg/mg lysate
    WB: 1/1000 - 1/5000. Predicted molecular weight: 64 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Activates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
    • Tissue specificity
      Highest expression in prostate, testis and colon.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated on serine residues when activated by CDC42/p21.
      Phosphorylated on tyrosine residues upon stimulation of FGFR2.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1142 antibody
      • p21 activated kinase 4 antibody
      • p21 Cdc42/Rac1-actiated kinase 4 antibody
      • P21 protein (Cdc42/Rac) activated kinase 4 antibody
      • p21(CDKN1A) activated kinase 4 antibody
      • p21-activated kinase 4 antibody
      • PAK 4 antibody
      • PAK-4 antibody
      • Pak4 antibody
      • PAK4_HUMAN antibody
      • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
      • Serine threonine kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK4 antibody
      • Serine/threonine-protein kinase PAK 4 antibody
      see all

    Images



    • Predicted band size : 64 kDa


      Detection of Human PAK4 by Western Blot following Immunoprecipitation. Samples: Whole cell lysate from HeLa (5 x 106) or Jurkat (25 x 106) cells. Antibody: Affinity purified rabbit anti-PAK4 (ab17974) used at 4 mcg for IP and at 1 mcg/ml for Western Blot. Detection: Chemiluminescence.

    References

    ab17974 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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