Overview

  • Product nameAnti-PAK4 antibody
    See all PAK4 primary antibodies
  • Description
    Rabbit polyclonal to PAK4
  • Tested applicationsSuitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Guinea pig, Dog, Pig, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide representing a portion of human p21(CDKN1A) activated kinase 4 encoded within exon 4 (Locuslink ID 10298).

  • Positive control
    • Whole cell lysate of Hela or Jurkat cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab17974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
WB
  • Application notes
    IP: 1 to 4 mcg/mg lysate
    WB: 1/1000 - 1/5000. Predicted molecular weight: 64 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionActivates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
    • Tissue specificityHighest expression in prostate, testis and colon.
    • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
      Contains 1 CRIB domain.
      Contains 1 protein kinase domain.
    • Post-translational
      modifications
      Autophosphorylated on serine residues when activated by CDC42/p21.
      Phosphorylated on tyrosine residues upon stimulation of FGFR2.
    • Information by UniProt
    • Database links
    • Alternative names
      • KIAA1142 antibody
      • p21 activated kinase 4 antibody
      • p21 Cdc42/Rac1-actiated kinase 4 antibody
      • P21 protein (Cdc42/Rac) activated kinase 4 antibody
      • p21(CDKN1A) activated kinase 4 antibody
      • p21-activated kinase 4 antibody
      • PAK 4 antibody
      • PAK-4 antibody
      • Pak4 antibody
      • PAK4_HUMAN antibody
      • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
      • Serine threonine kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK 4 antibody
      • Serine/threonine protein kinase PAK4 antibody
      • Serine/threonine-protein kinase PAK 4 antibody
      see all

    Anti-PAK4 antibody images



    • Predicted band size : 64 kDa


      Detection of Human PAK4 by Western Blot following Immunoprecipitation. Samples: Whole cell lysate from HeLa (5 x 106) or Jurkat (25 x 106) cells. Antibody: Affinity purified rabbit anti-PAK4 (ab17974) used at 4 mcg for IP and at 1 mcg/ml for Western Blot. Detection: Chemiluminescence.

    References for Anti-PAK4 antibody (ab17974)

    ab17974 has not yet been referenced specifically in any publications.

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