Overview

  • Product nameAnti-PAK4 antibody
    See all PAK4 primary antibodies
  • Description
    Rabbit polyclonal to PAK4
  • SpecificityThis antibody reacts with PAK4
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A 13 amino acid synthetic peptide from near the center of human PAK4

  • Positive control
    • SW480 cell lysate and human colon tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab62509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • FunctionActivates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • Tissue specificityHighest expression in prostate, testis and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1142 antibody
    • p21 activated kinase 4 antibody
    • p21 Cdc42/Rac1-actiated kinase 4 antibody
    • P21 protein (Cdc42/Rac) activated kinase 4 antibody
    • p21(CDKN1A) activated kinase 4 antibody
    • p21-activated kinase 4 antibody
    • PAK 4 antibody
    • PAK-4 antibody
    • Pak4 antibody
    • PAK4_HUMAN antibody
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
    • Serine threonine kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK4 antibody
    • Serine/threonine-protein kinase PAK 4 antibody
    see all

Anti-PAK4 antibody images

  • Lane 1 : Anti-PAK4 antibody (ab62509) at 1 µg/ml
    Lane 2 : Anti-PAK4 antibody (ab62509) at 2 µg/ml

    Lane 1 : SW480 cell lysate
    Lane 2 : SW480 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • Immunohistochemistry of PAK4 in paraffin embedded human colon tissue section with ab62509 antibody at 10 µg/ml.
  • ICC/IF image of ab62509 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62509, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of PAK4 in Human Colon cells using ab62509 at 20 ug/ml.

References for Anti-PAK4 antibody (ab62509)

This product has been referenced in:
  • Wen X  et al. ROCK1 and LIM Kinase Modulate Retrovirus Particle Release and Cell-Cell Transmission Events. J Virol 88:6906-21 (2014). Read more (PubMed: 24696479) »
  • Wang Z  et al. MiR-145 regulates PAK4 via the MAPK pathway and exhibits an antitumor effect in human colon cells. Biochem Biophys Res Commun : (2012). WB ; Human . Read more (PubMed: 22766504) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (SDS-PAGE 10%)
Sample Chinese Hamster Cell lysate - whole cell (CHO cells)
Specification CHO cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Sep 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"