Anti-PAK4 (phospho S474) antibody (ab125274)


  • Product nameAnti-PAK4 (phospho S474) antibody
    See all PAK4 primary antibodies
  • Description
    Rabbit polyclonal to PAK4 (phospho S474)
  • Specificityab125274 recognizes the phosphorylated peptide only. It recognises the PAK4 protein at a phosphorylation site of Serine 474 (~68kDa), or serine 602 of PAK5 (~90kDa) or Serine 560 of PAK6 (~80 kDa). ab125274 does not cross react to PAK1, PAK2 or PAK3.
  • Tested applicationsSuitable for: WB, IP, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow, Dog
  • Immunogen

    A synthetic peptide surrounding the epitope RKSLV-with a phosphorylation at Serine 474 of Human PAK4. This sequence is also identical within rat, mouse, chicken, cow and dog species.

  • Positive control
    • HEK293 cell lysate


Associated products


Our Abpromise guarantee covers the use of ab125274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
IP Use a concentration of 2 - 5 µg/ml.
ELISA Use a concentration of 0.01 - 0.1 µg/ml.


  • FunctionActivates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
  • Tissue specificityHighest expression in prostate, testis and colon.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    Autophosphorylated on serine residues when activated by CDC42/p21.
    Phosphorylated on tyrosine residues upon stimulation of FGFR2.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1142 antibody
    • p21 activated kinase 4 antibody
    • p21 Cdc42/Rac1-actiated kinase 4 antibody
    • P21 protein (Cdc42/Rac) activated kinase 4 antibody
    • p21(CDKN1A) activated kinase 4 antibody
    • p21-activated kinase 4 antibody
    • PAK 4 antibody
    • PAK-4 antibody
    • Pak4 antibody
    • PAK4_HUMAN antibody
    • Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs antibody
    • Serine threonine kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK 4 antibody
    • Serine/threonine protein kinase PAK4 antibody
    • Serine/threonine-protein kinase PAK 4 antibody
    see all

Anti-PAK4 (phospho S474) antibody images

  • All lanes : Anti-PAK4 (phospho S474) antibody (ab125274) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : HEK293 cell lysate with immunizing peptide

    Predicted band size : 64 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)

References for Anti-PAK4 (phospho S474) antibody (ab125274)

ab125274 has not yet been referenced specifically in any publications.

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