Specificityab125274 recognizes the phosphorylated peptide only. It recognises the PAK4 protein at a phosphorylation site of Serine 474 (~68kDa), or serine 602 of PAK5 (~90kDa) or Serine 560 of PAK6 (~80 kDa). ab125274 does not cross react to PAK1, PAK2 or PAK3.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 64 kDa).
Use a concentration of 2 - 5 µg/ml.
Use a concentration of 0.01 - 0.1 µg/ml.
FunctionActivates the JNK pathway. Plays a role in the reorganization of the actin cytoskeleton and in the formation of filopodia. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates ARHGEF2.
Tissue specificityHighest expression in prostate, testis and colon.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. Contains 1 CRIB domain. Contains 1 protein kinase domain.
Post-translational modificationsAutophosphorylated on serine residues when activated by CDC42/p21. Phosphorylated on tyrosine residues upon stimulation of FGFR2.