Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide from the C terminal region of Human PAK5.

  • Positive control
    • Human brain tissue; Rat brain cells extracts

Properties

Applications

Our Abpromise guarantee covers the use of ab110069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 81 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    The activated kinase acts on a variety of targets.
  • Tissue specificity
    Predominantly expressed in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • KIAA1264 antibody
    • MGC26232 antibody
    • p21 activated kinase 7 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 7 antibody
    • p21(CDKN1A) activated kinase 7 antibody
    • p21-activated kinase 5 antibody
    • p21-activated kinase 7 antibody
    • PAK 5 antibody
    • PAK 7 antibody
    • PAK-5 antibody
    • PAK-7 antibody
    • PAK5 antibody
    • PAK7 antibody
    • PAK7_HUMAN antibody
    • Protein kinase PAK5 antibody
    • Serine/threonine protein kinase PAK 7 antibody
    • Serine/threonine-protein kinase PAK 7 antibody
    see all

Images

  • All lanes : Anti-PAK5 antibody (ab110069) at 1/500 dilution

    Lane 1 : extracts from Rat brain cells
    Lane 2 : extracts from Rat brain cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 81 kDa
  • Immunohistochemical analysis of paraffin embedded Human brain tissue, using a 1/50 dilution of ab110069 with (right) or without (left) the immunizing peptide.

References

This product has been referenced in:
  • Aburatani T  et al. High expression of P21-activated kinase 5 protein is associated with poor survival in gastric cancer. Oncol Lett 14:404-410 (2017). Read more (PubMed: 28693183) »
  • Gu X  et al. Efficient inhibition of human glioma development by RNA interference-mediated silencing of PAK5. Int J Biol Sci 11:230-7 (2015). WB, IHC . Read more (PubMed: 25632266) »

See all 4 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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