Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK5
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human PAK5 aa 183-198 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    SEVKPLKSDFARFSAD


    Database link: Q9P286

  • Positive control
    • WB: PAK5 transiently transfected Cos-7 cell lysate. ICC/IF: SKNSH cell line. IHC-P: Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab37753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50.
WB 1/1000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).
ICC/IF 1/50.

Target

  • Function
    The activated kinase acts on a variety of targets.
  • Tissue specificity
    Predominantly expressed in brain.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt
  • Database links
  • Alternative names
    • EC 2.7.11.1 antibody
    • KIAA1264 antibody
    • MGC26232 antibody
    • p21 activated kinase 7 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 7 antibody
    • p21(CDKN1A) activated kinase 7 antibody
    • p21-activated kinase 5 antibody
    • p21-activated kinase 7 antibody
    • PAK 5 antibody
    • PAK 7 antibody
    • PAK-5 antibody
    • PAK-7 antibody
    • PAK5 antibody
    • PAK7 antibody
    • PAK7_HUMAN antibody
    • Protein kinase PAK5 antibody
    • Serine/threonine protein kinase PAK 7 antibody
    • Serine/threonine-protein kinase PAK 7 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFa-fixed paraffin-embedded sections) analysis of human brain tissue labelling PAK5 with ab37753. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • ab37753 stained SKNSH cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab37753 at 1/50 dilution) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-PAK5 antibody (ab37753) at 1/100 dilution

    Lane 1 : COS7 cells (negative control)
    Lane 2 : transiently transfected COS7 cells with PAK1
    Lane 3 : transiently transfected COS7 cells with PAK2
    Lane 4 : transiently transfected COS7 cells with PAK4
    Lane 5 : transiently transfected COS7 cells with PAK5
    Lane 6 : transiently transfected COS7 cells with PAK6


    Predicted band size : 81 kDa
    Observed band size : 81 kDa
    Additional bands at : 37 kDa,50 kDa,65 kDa. We are unsure as to the identity of these extra bands.

References

ab37753 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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