Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK6
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human PAK6 aa 131-146 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    QSPQSERTDPHGLYLS

  • Positive control
    • WB: Transiently transfected Cos-7 cell lysate. ICC/IF: LoVo cells. IHC-P: Human Cerebral Cortex (normal) tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37749 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    The activated kinase acts on a variety of targets.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDKN1A activated kinase 6 antibody
    • EC 2.7.1.37 antibody
    • p21 activated protein kinase 6 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 6 antibody
    • p21(CDKN1A) activated kinase 6 antibody
    • p21-activated kinase 6 antibody
    • p21activated kinase 6 antibody
    • PAK 5 antibody
    • PAK 6 antibody
    • PAK-5 antibody
    • PAK-6 antibody
    • PAK5 antibody
    • Pak6 antibody
    • PAK6_HUMAN antibody
    • Serine threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK6 antibody
    • Serine/threonine-protein kinase PAK 6 antibody
    see all

Images

  • IHC image of PAK6 staining in Human cerebral Cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37749, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  • ab37749 stained LoVo cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab37749 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-PAK6 antibody (ab37749)

    Lane 1 : Negative control
    Lane 2 : PAK1-expressing cell lysate
    Lane 3 : PAK2-expressing cell lysate
    Lane 4 : PAK4-expressing cell lysate
    Lane 5 : PAK5-expressing cell lysate
    Lane 6 : PAK6-expressing cell lysate


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
    Lanes 2-6 contain lysates from transiently transfected COS7 cells.

References

ab37749 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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