Overview

  • Product name
  • Description
    Rabbit polyclonal to PAK6
  • Tested applications
    Suitable for: WB, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (Human) of 13 amino acids from near the centre of PAK6

  • Positive control
    • Raji cells

Properties

Applications

Our Abpromise guarantee covers the use of ab62511 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).Can be blocked with PAK6 peptide (ab209067).
ICC Use a concentration of 10 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.

Target

  • Function
    The activated kinase acts on a variety of targets.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDKN1A activated kinase 6 antibody
    • EC 2.7.1.37 antibody
    • p21 activated protein kinase 6 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 6 antibody
    • p21(CDKN1A) activated kinase 6 antibody
    • p21-activated kinase 6 antibody
    • p21activated kinase 6 antibody
    • PAK 5 antibody
    • PAK 6 antibody
    • PAK-5 antibody
    • PAK-6 antibody
    • PAK5 antibody
    • Pak6 antibody
    • PAK6_HUMAN antibody
    • Serine threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK6 antibody
    • Serine/threonine-protein kinase PAK 6 antibody
    see all

Images

  • Lane 1 : Anti-PAK6 antibody (ab62511) at 1 µg/ml
    Lane 2 : Anti-PAK6 antibody (ab62511) at 2 µg/ml
    Lane 3 : Anti-PAK6 antibody (ab62511) at 4 µg/ml

    Lane 1 : Raji lysate
    Lane 2 : Raji lysate
    Lane 3 : Raji lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa
  • Immunocytochemistry of Raji cells labelled with ab62511 at 10 µg/ml.
  • Immunofluorescence of PAK6 in Raji cells using ab62511 at 10 ug/ml.

References

This product has been referenced in:
  • Bastea LI  et al. Protein kinase D-mediated phosphorylation at Ser99 regulates localization of p21-activated kinase 4. Biochem J 455:251-60 (2013). Read more (PubMed: 23841590) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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