Anti-PAK6 (phospho S165) antibody (ab41432)

Overview

  • Product name
    Anti-PAK6 (phospho S165) antibody
    See all PAK6 primary antibodies
  • Description
    Rabbit polyclonal to PAK6 (phospho S165)
  • Specificity
    Phospho-Ser165-specific. The antibody detects a 75 kDa band on SDS-PAGE immunoblots of human PAK6 recombinant protein phosphorylated with ERK2. This reactivity is specifically blocked by pre-incubation with phospho-Ser165-PAK6 peptide and also by treatment with lambda phosphatase.
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-Ser165-PAK6 peptide (coupled to carrier protein) corresponding to amino acids around serine 165 in human PAK6. The sequence used has two amino acid differences compared to rat and mouse PAK6.

  • General notes
    Do not aliquot.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was cross-adsorbed to unphosphorylated-Ser165-PAK6 peptide, then affinity purified using phospho-Ser165-PAK6 peptide (immunogen).
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab41432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
ELISA 1/2000.

Target

  • Function
    The activated kinase acts on a variety of targets.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 CRIB domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated when activated by CDC42/p21.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDKN1A activated kinase 6 antibody
    • EC 2.7.1.37 antibody
    • p21 activated protein kinase 6 antibody
    • p21 protein (Cdc42/Rac)-activated kinase 6 antibody
    • p21(CDKN1A) activated kinase 6 antibody
    • p21-activated kinase 6 antibody
    • p21activated kinase 6 antibody
    • PAK 5 antibody
    • PAK 6 antibody
    • PAK-5 antibody
    • PAK-6 antibody
    • PAK5 antibody
    • Pak6 antibody
    • PAK6_HUMAN antibody
    • Serine threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK 6 antibody
    • Serine/threonine protein kinase PAK6 antibody
    • Serine/threonine-protein kinase PAK 6 antibody
    see all

Images

  • Lanes 1 - 4 : Anti-PAK6 antibody (ab41431) at 1/1000 dilution
    Lanes 5 - 8 : Anti-PAK6 (phospho S165) antibody (ab41432) at 1/1000 dilution

    Lane 1 : Human PAK6 recombinant protein phosphorylated by ERK2
    Lane 2 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
    Lane 3 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific peptide
    Lane 4 : Human PAK6 recombinant protein phosphorylated by ERK2 with PAK6 N-terminal peptide (ab41431 immunogen)
    Lane 5 : Human PAK6 recombinant protein phosphorylated by ERK2
    Lane 6 : Human PAK6 recombinant protein phosphorylated by ERK2; blot exposed to lambda phosphatase before probing
    Lane 7 : Human PAK6 recombinant protein phosphorylated by ERK2 with phospho-S165-PAK6 peptide (ab41432 immunogen)
    Lane 8 : Human PAK6 recombinant protein phosphorylated by ERK2 with non-specific phospho-serine peptide


    Predicted band size : 75 kDa
    Observed band size : 75 kDa

References

ab41432 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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