Recombinant
RabMAb

Anti-Palladin antibody [EPR9460(B)] (ab154827)

Overview

  • Product name
    Anti-Palladin antibody [EPR9460(B)]
    See all Palladin primary antibodies
  • Description
    Rabbit monoclonal [EPR9460(B)] to Palladin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues near the N terminus of Human Palladin (UniProt Q8WX93).

  • Positive control
    • HeLa, 293T, PC3 and Caco-2 cell lysates; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab154827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 151 kDa.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Relevance
      Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific subcellular foci (from SwissProt).
    • Cellular localization
      Cytoplasm, cytoskeleton. Note: Localizes to stress fibers and Z disks. Cell junction, focal adhesion. Cell projection, ruffle. Cell projection, lamellipodium.
    • Database links
    • Alternative names
      • CGI 151 antibody
      • CGI151 antibody
      • KIAA0992 antibody
      • MYN antibody
      • Myoneurin antibody
      • pa antibody
      • Palladin cytoskeletal associated protein antibody
      • Palld antibody
      • PNCA1 antibody
      • Sarcoma antigen NY SAR 77 antibody
      • SIH002 antibody
      • Stx13bp1 antibody
      see all

    Images

    • All lanes : Anti-Palladin antibody [EPR9460(B)] (ab154827) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 151 kDa

    • Flow cytometric analysis of permeabilized HeLa cells labeling Palladin with ab154827 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    ab154827 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab154827.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up