Overview

  • Product name
  • Description
    Rabbit polyclonal to PAM
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 146-195 (PKGVGFRVGG ETGSKYFVLQ VHYGDISAFR DNNKDCSGVS LHLTRLPQPL) of Human PAM (NP_000910).

  • Positive control
    • 721_B cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab105069 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 108 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
  • Sequence similarities
    In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
    In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
    Contains 5 NHL repeats.
  • Cellular localization
    Membrane and Secreted. Secreted from secretory granules.
  • Information by UniProt
  • Database links
  • Alternative names
    • AMD_HUMAN antibody
    • PAL antibody
    • PAM antibody
    • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
    • Peptidyl alpha amidating enzyme antibody
    • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
    • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
    • Peptidylamidoglycolate lyase antibody
    • Peptidylglycine 2 hydroxylase antibody
    • Peptidylglycine alpha amidating monooxygenase antibody
    • Peptidylglycine alpha hydroxylating monooxygenase antibody
    • PHM antibody
    see all

Images

  • Anti-PAM antibody (ab105069) at 1 µg/ml + 721_B cell lysate at 10 µg

    Predicted band size : 108 kDa

References

ab105069 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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