Overview

  • Product name
  • Description
    Goat polyclonal to PAM
  • Specificity
    This antibody is expected to recognise all reported isoforms (NP_000910.2; NP_620121.1; NP_620176.1 and NP_620177.1).
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    DQEKEDDGSESEE

    , corresponding to C terminal amino acids 947-959 of Human PAM (NP_000910.2; NP_620121.1; NP_620176.1; NP_620177.1)

  • Positive control
    • Human kidney lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab77592 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notes
    Peptide ELISA: Antibody detection limit dilution 1/4000.
    WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 108 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
    • Sequence similarities
      In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
      In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
      Contains 5 NHL repeats.
    • Cellular localization
      Membrane and Secreted. Secreted from secretory granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMD_HUMAN antibody
      • PAL antibody
      • PAM antibody
      • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
      • Peptidyl alpha amidating enzyme antibody
      • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
      • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
      • Peptidylamidoglycolate lyase antibody
      • Peptidylglycine 2 hydroxylase antibody
      • Peptidylglycine alpha amidating monooxygenase antibody
      • Peptidylglycine alpha hydroxylating monooxygenase antibody
      • PHM antibody
      see all

    Images

    References

    ab77592 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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