Overview

  • Product name
    Anti-PAM antibody [EPR2643(2)]
    See all PAM primary antibodies
  • Description
    Rabbit monoclonal [EPR2643(2)] to PAM
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human PAM (C terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal heart tissue lysate and PC-3 cell lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Bifunctional enzyme that catalyzes 2 sequential steps in C-terminal alpha-amidation of peptides. The monooxygenase part produces an unstable peptidyl(2-hydroxyglycine) intermediate that is dismutated to glyoxylate and the corresponding desglycine peptide amide by the lyase part. C-terminal amidation of peptides such as neuropeptides is essential for full biological activity.
    • Sequence similarities
      In the C-terminal section; belongs to the peptidyl-alpha-hydroxyglycine alpha-amidating lyase family.
      In the N-terminal section; belongs to the copper type II ascorbate-dependent monooxygenase family.
      Contains 5 NHL repeats.
    • Cellular localization
      Membrane and Secreted. Secreted from secretory granules.
    • Information by UniProt
    • Database links
    • Alternative names
      • AMD_HUMAN antibody
      • PAL antibody
      • PAM antibody
      • Pancreatic peptidylglycine alpha amidating monooxygenase antibody
      • Peptidyl alpha amidating enzyme antibody
      • Peptidyl alpha hydroxyglycine alpha amidating lyase antibody
      • Peptidyl-alpha-hydroxyglycine alpha-amidating lyase antibody
      • Peptidylamidoglycolate lyase antibody
      • Peptidylglycine 2 hydroxylase antibody
      • Peptidylglycine alpha amidating monooxygenase antibody
      • Peptidylglycine alpha hydroxylating monooxygenase antibody
      • PHM antibody
      see all

    Images

    • Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/10000 dilution (purified) + Human fetal heart tissue lysate at 20 µg

      Secondary
      HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size : 108 kDa
      Observed band size : 108 kDa
      Blocking buffer: 5% NFDM/TBST.
      Dilution buffer: 1%BSA/TBST.
    • All lanes : Anti-PAM antibody [EPR2643(2)] (ab109175) at 1/1000 dilution (unpurified)

      Lane 1 : Human fetal heart tissue lysate
      Lane 2 : PC-3 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 108 kDa

    References

    This product has been referenced in:
    • Simpson PD  et al. Striking Oxygen Sensitivity of the Peptidylglycine a-Amidating Monooxygenase (PAM) in Neuroendocrine Cells. J Biol Chem 290:24891-901 (2015). WB ; Human . Read more (PubMed: 26296884) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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