Anti-PAMCI antibody (ab150853)
Key features and details
- Rabbit polyclonal to PAMCI
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-PAMCI antibody -
Description
Rabbit polyclonal to PAMCI -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to amino acids 357-435 of Human PAMCI
-
Positive control
- Human breast tissue, RT4 cell lysate
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa.
|
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 50 kDa. |
Target
-
Function
May play a role in regulating vesicuar trafficking in cells. -
Sequence similarities
Contains 1 Ras-associating domain. -
Cellular localization
Endosome. Accumulates on perinuclear endosomes. - Information by UniProt
-
Database links
- Entrez Gene: 9182 Human
- Entrez Gene: 237504 Mouse
- Entrez Gene: 65053 Rat
- Omim: 610383 Human
- SwissProt: O75901 Human
- SwissProt: Q8K342 Mouse
- SwissProt: O88869 Rat
- Unigene: 527881 Human
see all -
Alternative names
- P CIP1 antibody
- P-CIP1 antibody
- PAM COOH-terminal interactor protein 1 antibody
see all
Images
-
All lanes : Anti-PAMCI antibody (ab150853) at 1/100 dilution
Lane 1 : NIH-3T3 cell lysate (Mouse embryonic cells)
Lane 2 : NBT-II cell lysate (Rat Wistar bladder tumour cells)
Predicted band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast tissue labeling PAMCI with ab150853 at 1/200 dilution
-
All lanes : Anti-PAMCI antibody (ab150853) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : U251 MG cell lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver lysate
Lane 5 : Human tonsil lysate
Predicted band size: 50 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab150853 has not yet been referenced specifically in any publications.